dark-field microscopy


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dark-field microscopy

a microscope technique that involves creating a dark background to the subject to be observed under the microscope and viewing this by reflected light; the object thus appears light on a dark background.
References in periodicals archive ?
Her Bochum team has now brought it together with the so-called dark-field microscopy. The latter allows the chemists to visualize the particles in real time as coloured dots.
Direct examination with dark-field microscopy of specimens from a patient with agammaglobulinemia who had Spiroplasma apis infection, France.
Identification of spirochetes in bone lesions with dark-field microscopy, silver stain, immunoperoxidase stain, or polymerase chain reaction is uncommon and occurs even less in late-stage disease [2, 5].
(21.) "Dark-Field Microscopy," Wikipedia (2017), https:// en.wikipedia.org/wi ki/Dark-field_microscopy (accessed 29 July 2017).
The advantages and limitations of TEM and atomic force microscopy, micro-Raman spectroscopy, classical optical and high-resolution dark-field microscopy were described with respect to their relevance in in vitro and systemic toxicity studies.
For instance, our lab used a SIT camera for imaging of sliding microtubules, using dark-field microscopy (e.g., Vale and Toyoshima, 1988) and other low-light applications such as fluorescence imaging.
While syphilis can be diagnosed by direct detection of the treponemal spirochete under dark-field microscopy, it is usually identified by one of 2 quick and inexpensive serologic screening tests: an RPR or a venereal disease research laboratory (VDRL) test.
Direct Evidence: Dark-Field Microscopy: Microscopy of blood is of value only during the first few days of the acute illness.
Dark-field microscopy is also helpful in revealing the refractile character of silicone.
(10, 11) Other significant disadvantages of dark-field microscopy include the absence of species identification and the lack of guidance concerning the choice of an appropriate antibiotic.
* Perform dark-field microscopy to detect small, spiral spirochete with corkscrew-rotation motility (http://www.cdc.gov/std).
For dark-field microscopy a quadratic enhancement is expected, Due to coherent build-up of scattered fields.