Direct examination with dark-field microscopy
of specimens from a patient with agammaglobulinemia who had Spiroplasma apis infection, France.
Identification of spirochetes in bone lesions with dark-field microscopy
, silver stain, immunoperoxidase stain, or polymerase chain reaction is uncommon and occurs even less in late-stage disease [2, 5].
(21.) "Dark-Field Microscopy
," Wikipedia (2017), https:// en.wikipedia.org/wi ki/Dark-field_microscopy (accessed 29 July 2017).
The advantages and limitations of TEM and atomic force microscopy, micro-Raman spectroscopy, classical optical and high-resolution dark-field microscopy
were described with respect to their relevance in in vitro and systemic toxicity studies.
For instance, our lab used a SIT camera for imaging of sliding microtubules, using dark-field microscopy
(e.g., Vale and Toyoshima, 1988) and other low-light applications such as fluorescence imaging.
While syphilis can be diagnosed by direct detection of the treponemal spirochete under dark-field microscopy
, it is usually identified by one of 2 quick and inexpensive serologic screening tests: an RPR or a venereal disease research laboratory (VDRL) test.
Direct Evidence: Dark-Field Microscopy
: Microscopy of blood is of value only during the first few days of the acute illness.
is also helpful in revealing the refractile character of silicone.
(10, 11) Other significant disadvantages of dark-field microscopy
include the absence of species identification and the lack of guidance concerning the choice of an appropriate antibiotic.
* Perform dark-field microscopy
to detect small, spiral spirochete with corkscrew-rotation motility (http://www.cdc.gov/std).