Este trabajo presenta los resultados de un experimento economico de campo contextualizado cuyo objetivo es explorar los efectos de las diferencias en productividad biologica entre AMERB sobre el comportamiento infractor cuando, en el contexto de DUTP
, existe una regulacion de cuotas, y la fiscalizacion externa es insuficiente para inducir perfecto cumplimiento.
Several tests such as TdT (terminal deoxyribonucleotidyl transferase)-mediated dUTP
nick-end labeling (TUNEL) assay, aniline blue straining, chromomycin A3 and acridine orange (AO) staining have been described to determine the extent of DNA damage.
TDT is used to transfer the dUTP
labeled with fluorescent marker (e.
TUNEL (Terminal deoxynucleotidyl Transferase-mediated dUTP
Nick End Labeling) has been done by mammary tissue were sliced, then drooped with 50ul of TUNNEL labeling mix (consisting of 5ulenzymeterminal deoxynucleotydil transferase and 45ulfluorescein-dUTP
), incubated at 37 [degrees]C for 30 minutes in the moist chamber.
The 18S rDNA and 5S rDNA probes were labeled by nick translation with biotin-14-dATP, or 11-digoxigenine dUTP
following the manufacturer's (Bionick TM Labelling System-Gibco.
Terminal deoxynucleotidyl transferase dUTP
nick end labeling (TUNEL) assay: TUNEL assay was performed using the DeadEnd Fluorometric TUNEL System kit as instructed by the manufacturer (Promega, Madison, WI, USA).
Posteriormente, los cortes fueron incubados en una mezcla conteniendo la enzima deoxinucleotidil transferasa terminal (TdT), dUTP
unida a una molecula de deteccion como digoxigenina y dATP en camara humeda a 37 [degrees]C durante una hora y luego se lavo con PBS.
Terminal deoxynucleotidyl transferase mediated deoxyuridine Triphosphate-Nick End Labeling (TUNEL) assay quantifies the integration of the flurochrome or biotin labeled dUTP
at single and double-strand DNA breakages in a reaction catalyzed by the Terminal deoxynucleotidyl Transferase (TdT) enzyme that is not dependent on the template.
On the basis of the terminal deoxynucleotidyl transferase-mediated dUTP
nick-end labeling (TUNEL) assay, it is reported that 50-80% of liver endothelial cells and hepatocytes die through apoptosis during the first 3-6 h of reperfusion .
The fragments were fixed, embedded in paraffin, and cut into serial 5-[micro]m-thick sections that were used for confirmation of the diagnosis, immunohistochemistry for the detection of TNF-[alpha], IFN-[gamma], Bcl-2, Fas, and iNOS, and detection of apoptosis by the terminal deoxynucleotidyl transferase-mediated dUTP
nick-end labeling (TUNEL) technique.
TUNEL (terminal dUTP
nick-end labeling) assays were performed at baseline and at week 4 to evaluate the percentage of sperm DNA fragmentation.