dTTP


Also found in: Dictionary, Acronyms, Wikipedia.

dTTP

Abbreviation for thymidine 5'-triphosphate.
Farlex Partner Medical Dictionary © Farlex 2012

dTTP

(dē′tē′tē′pē′)
n.
One of the two pyrimidine nucleotides used to synthesize DNA.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.
References in periodicals archive ?
(8) Shari Laster, "Segmenting the Government Information Corpus." DttP: Documents to the People 43, no.1 (Spring 2015): 15-16.
Labeling was obtained using a PCR procedure with the following dNTP concentrations: 200 [micro]M dATP, 200 [micor]M dCTP, 200 [micro]M dGTP, 150 [micro]M dTTP, and 50 [micro]M dig-11-dUTP (Roche Molecular Biochemicals).
Condicoes de amplificacao do DNA: Preparou-se uma mistura de reagentes em um microtubo para capacidade para 200 [micro]L que consistiu em 1, 5x de tampao Taq, 4 mM de Mg[Cl.sub.2], 0, 2 [micro]M de cada oligonucleotideo (G1 e G2), 100 [micro]M de dNTP (dATP, dTTP, dCTP e dGTP) e 2,5U, de Taq DNA Polimerase (Ludiwing [R]).
For PCR amplification of the CYP3A43 rs501275 polymorphism we used the primers F 5'-GTCAATGGCAATTTTCTGTT-3' and R 5'-CTGTCTTCACAAACCAGATG-3' in a 50 [micro]l reaction mix containing 50 ng of genomic DNA, 120 ng of each of the forward (F) and reverse (R) primers, 200 [micro]M of dATP, dCTP, dGTP and dTTP, 1.5 mM Mg[Cl.sup.2], 1 [micro]l 10 x Taq DNA polymerase buffer, and 0.5 U Taq DNA polymerase.
Polymerase Chain Reactions (PCR) were carried out in a final volume of 25 [micro]l containing 20 ng template DNA, 100[micro]M each dATP, dTTP, dGTP & dCTP, 20 ng of decanucleotide primers, 1.5mM Mg[Cl.sub.2], 1 x taq buffer (Fermantas) [10mM Tris-HCl (pH 9.0), 50mM KCl, 0.01% gelatin] and 0.5 U Taq DNA polymerase (Fermentas).
Standard Polymerase Chain Reaction (PCR) was carried out in 25[micro]l final volume containing 50 ng/[micro]l of genomic DNA; 1X Ammonium buffer; 1.25 mM of Mg[Cl.sub.2]; 12.5 pmol of each primer pair; 0.04 mM of each deoxynucleotide (dTTP, dGTP, dCTP, and dATP); 0.5 U of Taq Recombinant polymerase (Fermentas Life Sciences, USA).
ABBREVIATIONS: bDNA = Branched DNA; DNA = Deoxyribonucleic Acid; dNTP = Deoxyribonucleotides; dATP = Deoxyadenosine Triphosphate; dCTP = Deoxycytidine Triphosphate; dGTP = Deoxyguanosine Triphosphate; dTTP = Deoxythymidine Triphosphate; FDA = Food and Drug Administration; FRET = Fluorescence Resonance Energy Transfer; MRSA = Methicillin Resistant Staph aureus; MTB = Mycobacterium tuberculosis; NASBA = Nucleic Acid Sequence Based Amplification; PCR = Polymerase Chain Reaction; PFGE = Pulsed-Field Gel Electrophoresis; RT-PCR = Reverse Transcription Polymerase Chain Reaction; Taq = Thermus aquaticus; TMA = Transcription-Mediated Amplification; VRE = Vancomycin Resistant Enterococcus.
Omission of dATP, dTTP, or dGTP during the telomerase extension step of the TRAP reaction resulted in no elongation products being amplified; however, omission of dCTP did not affect the formation of a ladder pattern of telomerase products (data not shown).
A reaction mixture of 25 ml containing 10 mM Tris-HCl (pH 8.3 at 25[degrees]C), 50 mM KCl, 3 mM Mg[Cl.sub.2], 0.1 mM each of dATP, dGTP, dTTP and dCTP, one unit of Taq DNA polymerase (Perkin Elmer, Norwalk, Conn.), 0.001% gelatin (Sigma, St-Louis, Mo.), 25 ng of template DNA and 30 ng of primer was prepared and overlaid with two drops of mineral oil in order to avoid evaporation.
El segmento de la region promotora del gen de la IL-10 entre las posiciones -1120 y -533, se amplifico por la reaccion en cadena de la polimerasa (PCR), realizada en el termociclador (iCicler, Bio Rad), en un volumen de reaccion de 50 [micron]l, asi: 250 ng de ADN, 20 mM Tri HCl, pH 8,0, 100 mM de KCl (Corpogen, Colombia), 2 mM Mg[Cl.sub.2], 200 [micron]M de cada dGTP, dATP, dTTP, dCTP (Promega, Madison, WI), O,5 [micron]M de cada iniciador (5'-ATCCAAGACAACACTA CTAA-3' y 5'-AAATATCCTCA AAGTTCC-3') y 2,5 unidades de Taq polimerasa (Corpogen, Colombia).
50 mM KCl, and 0.1% Triton X-100], 1 unit of Taq polymerase (Banglore Genei), 200 uM (each) deoxynucleoside triphosphate (dATP, dCTP, dGTP, and dTTP) (Banglore Genei), 10 pmol of each primer, 5 [mu]l of sample DNA, and ultra pure sterile water added to 25 [mu]l.