dNTP


Also found in: Acronyms.

dNTP

Deoxyribonucleotide triphosphate. A generic term referring to the four deoxyribonucleotides: dATP, dCTP, dGTP and dTTP.
References in periodicals archive ?
EGCG, EA, or RA treatment for 24 h each caused a significant imbalance of dNTPs in HL-60 cells, which was reminiscent of the imbalances caused by the RR inhibitors hydroxyurea and gemcitabine (Hakansson et al.
Pyrosequencing, with its low coefficient of variation, is inherently more quantitative: Different dNTPs generate similar peak heights following single incorporation events, and the peak height generated from incorporating 3 dNTPs is 3 times the signal from incorporating one of the same dNTPs.
25 [micro]mol/L dNTPs and LCGreen Plus, 50 blinded samples, including trisomy 13, 18, and 21 and wild type, were amplified using DNA ranging from 10-200 ng.
This binding is essential in maintaining the structure and reactivity of the dNTPs (and thus the reason why metal ion chelating agents can be effective inhibitors of PCR or other polymerase-based amplification methods).
Reduced extension rates were likely caused by lower available dNTPs due to incomplete conversion of the heatactivated nucleotides.
The environment needs to be maintained at physiological pH (usually achieved by buffering agents) and needs to include a supply of divalent cations--most frequently Mg+-1--which act in making the dNTPs chemically reactive.
2+] ions and pyrophosphate ions progressively produced by the incorporation of dNTPs into the growing DNA strand precipitates in the reaction mixture.
CORRIGENDUMVersion 109 Jun 2016For Item 2, vendors responses were in different measurements which does not match our requirement- 2 units of dNTPs mix solution which contains 10mM (2ml).
We speculated that lowering the concentrations of the input dNTPs renders the ASD-PCR assay specificity virtually independent from the amount of the input DNA template.
2], 3 U of AmpliTaq Gold DNA polymerase (Applied Biosystems), 200 nM each primer, 1 mM dNTPs (Amersham Pharmacia Biotech), and 50 ng of the genomic DNA.
The DNA polymerase in a few tissues can catalyze the synthesis of DNA using only a primer and four dNTPs without a template (27), which is accompanied by release of [PP.