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When one of these is incorporated in place of a regular dCTP, the growing nascent DNA chain terminates, as there is no 3'-OH for the polymerase to extend from.
2]), 200 [micro]M dNTPs (dATP, dTTP, dCTP and dGTP), and 0.
2] (Invitrogen, USA), 200 [micro]M of dNTP (Invitrogen, USA) containing each of dATP, dCTP, dGTP, dTTP the PCR primers, 2.
5 mmol/L each of dATP, dCTP, dGTP, and 5 mmol/L of dUTP; Applied Biosystems, Foster City, CA, USA), 5 [micro]L of Mg[Cl.
5 mM of each dATP, dTTP, dCTP, and dGTP (ABgene[R]); and 5 [micro]L of DNA.
5 [micro]l dNTP mix (10 mM each of dATP, dCTP, dGTP and dTTP), 1.
1% Triton X-100], 1 unit of Taq polymerase (Banglore Genei), 200 uM (each) deoxynucleoside triphosphate (dATP, dCTP, dGTP, and dTTP) (Banglore Genei), 10 pmol of each primer, 5 [mu]l of sample DNA, and ultra pure sterile water added to 25 [mu]l.
2], dNTPs (dATP, dGTP, dCTP, dTTP, 200 [micro]M each), and 5% DMSO.
2 mM each dATP, dCTP, dGTP, and dTTP, 1 [micro]M each forward and reverse primer, 0.
A poly(dC) tail was added to the 3'-terminus of the purified cDNA using dCTP and terminal deoxynucleotidyl transferase, and the cDNA region corresponding to the 5'-end of the mRNA was amplified by two successive rounds of PCR using additional gene-specific primers (GSP2, 5'-TACTCTTATTTCGTCGTGGTCC; GSP3, 5'-ACAGCAATCCAGAATTCACGG), together with anchor primers supplied by the manufacturer.
The treated gas and the condensate will be pumped from the DCTP into the national grid through two pipelines, each about 40 km.
2 mM each of dATP, dCTP, dGTP, dTTP, precipitant, and red dye for electrophoresis.
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