The partitioning of metals among the (1) nuclei and brown bodies; (2) lysosomes and mitochondria; (3) microsomes; and (4) soluble cytosolic
fraction is presented in Table 2.
Overproduction of FeSOD interferes with signal pathways, leading to induction of cytosolic
Cu/ZnSOD during salt stress.
There was no correlation between alcohol dehydrogenase activity and age, whether expressed as activity per mg cytosolic
protein (Figures 1 and 2) or per gram wet weight liver (Figures 3 and 4).
The 65-kDa cytosolic
protein associated with warm temperature acclimation in goldfish, Carassius auratus.
Since TLR4 often signals through HMGB1 to mediate inflammation [18, 19], we also measured cytosolic
levels of HMGB1 in the mice.
Effect of Thapsigargin on Cytosolic
Calcium in Differentiating Neurons and Effect of Calcium Addition on Thapsigargin-Primed Cells.
To explore the possible inhibitory effect of DNAJB6(S) on Bax activity, pDNAJB6(S) cells were treated with or without 500 pM [MPP.sup.+] for 48 h, and the LN18 cell, total lysate, and mitochondrial and cytosolic
fractions were isolated and analyzed by western blotting.
Orum Therapeutics is a private biotech company developing a new class of therapeutic antibodies targeting cytosolic
proteins for unmet medical needs in cancer and rare diseases.
Accordingly, western blot results did not reveal significant changes in NF-[kappa]B levels after 12 h of PM2.5 stimulation compared with baseline levels, but significantly decreased cytosolic
levels of NF-[kappa]B and increased nuclear levels were observed at 24 and 48 h of PM2.5 stimulation (Figure 4(a)).
By detecting cytosolic
DNA, STING traffics to the lysosome, where it induces membrane permeabilization and subsequent [K.sup.+] efflux that activates NLRP3-associated inflammasomes.
The data generated from the company's pre-clinical proof of concept demonstrated that CPABs can penetrate cancer cells and act on targets within the cytosolic
space to induce a therapeutic effect (induce apoptosis and death of the cancer cells).
The lysine (K) residues K5 and K12 on most newly synthesized cytosolic
histones type H4 are acetylated (Burgess and Zhang 2013).