31 EID50 was inoculated and the cell culture flasks were incubated at 37oC in the presence of 5% CO2 in a CO2 incubator and observed for cytopathogenic effects.
The complete and clear cytopathogenic effects (CPEs) were observed in passage 4 at 48 h P.
The antiviral activity of oral drops and dry extract was measured with plaque-reduction assays (Cooper 1955) in plaque forming units (PFU) for FluA and pFluA, Para 3, RSV, HRV 14, CA9 or with the analyses of a
cytopathogenic effect (CPE) for Adeno 5.
Determination of virus-induced
cytopathogenic effects and virus titres revealed that EPs [R] 7630 at concentrations up to 100 [micro]g/ml interfered with replication of seasonal influenza A virus strains (H1N1, H3N2), respiratory syncytial virus, human coronavirus, parainfluenza virus, and coxsackie virus but did not affect replication of highly pathogenic avian influenza A virus (H5N1), adenovirus, or rhinovirus.
The bottom panel indicates the approximate percentage of cells exhibiting
cytopathogenic effects in cultures at the corresponding intervals after infection.
Determination of virus-induced
cytopathogenic effects and virus titres revealed that EPs[R] 7630 at concentrations up to 100 [micro]g/ml interfered with replication of seasonal influenza A virus strains (H1N1, H3N2), respiratory syncytial virus, human coronavirus, parainfluenza virus, and coxsackie virus but did not affect replication of highly pathogenic avian influenza A virus (H5N1), adenovirus, or rhinovirus.
during the growth or the stationary phase) different
cytopathogenic effects might be observed.
Routine examination for
cytopathogenic effects using phase-contrast microscopy was performed following Hay's haemadsorption protocol (Hay, 1992).
Of the 616 samples, 238 induced
cytopathogenic effects in human cells, of which 20 also induced
cytopathogenic effects in L20B cells; the latter samples were confirmed by reverse transcription PCR molecular testing (7) to contain PV strains.
To measure the inhibitory effect on the viral
cytopathogenic effects (CPE) in cell culture, nebrodeolysin was exposed to different cell cultures that were infected by a variety of DNA and RNA viruses.
