We would like to thank the faculty and residents in the Family Medicine Center and OB/GYN Clinic who assisted with specimen collection, Jim Best for processing and interpreting cytologic specimens
, Aisha Lavin for data management assistance, and April Dean for manuscript preparation.
Some important technical aspects need to be considered for successful FISH on cytologic specimens
(7): to prevent cell loss during pretreatment and DNA denaturation, the use of adhesive slides with electrostatic positive charge is recommended.
Residual cells from the liquid-based cytologic specimen
and cells in the STM tube were then tested for carcinogenic HPV using the HC II and HCT HPV assays, respectively.
5,6) Several recent articles have discussed the preanalytic variables in cytologic specimens
and the need for standardization to validate and evaluate molecular testing in lung cancer using a variety of cytologic preparations.
If IHC is regularly done on cytologic specimens
that are not processed in the same manner as the tissues used for assay validation (eg, alcohol-fixed cell blocks, air-dried smears, formalin-postfixed specimens), laboratories should test a sufficient number of such cases to ensure that assays consistently achieve expected results.
4 389 Written procedure includes 697 specification(s) for validating IHC tests performed on cytologic specimens
Yes, for both predictive and 254 36.
Detection of EGFR mutations in archived cytologic specimens
of non-small cell lung cancer using high-resolution melting analysis.
not only provide a method for immediate confirmation of needle placement within a lesion, but also provide cytologic detail that is sometimes superior to that seen in core biopsies.
ThinPrep processor: clinical trials demonstrate an increased detection rate of abnormal cervical cytologic specimens
The authors conclude, "We have demonstrated the ThinPrep methanol fixation fluid-based collection of cervical cytologic specimens
preserves cellular contents that are useful for detailed molecular analysis.
Similar to core biopsies, low tumor content or DNA quality may be encountered in cytologic specimens
despite safeguards in tissue acquisition, preparation, and use.
All of these will be used for exploration, and because of their controlled manipulability, the physician will be able to obtain cytologic specimens
with instruments inserted through them.