Maternal and fetal serum levels of caspase-cleaved fragments of cytokeratin-18
in intrahepatic cholestasis of pregnancy.
Additional biomarkers associated with NASH and liver damaged were also reduced by IMM-124E including aspartate transaminase and cytokeratin-18
. The primary objective of the study was to evaluate the safety and efficacy of IMM-124E, at two oral dosage levels as compared with a placebo and provide proof of concept in human subjects for its unique Mechanism of Action.
fragment levels as noninvasive biomarkers for nonalcoholic steatohepatitis: a multicenter validation study," Hepatology, vol.
Gonzalez-Rivero et al., "Serum levels of caspase-cleaved cytokeratin-18
and mortality are associated in severe septic patients: pilot study," PLoS One, vol.
In the immunohistochemical analysis, the epithelial membrane antigen, pancytokeratin, was diffuse positive and cytokeratin-18
was positive (Figure 3), NSE was focal positive (Figure 4).
Wong et al., "Systematic reviewwith meta-analysis: non-invasive assessment of non-alcoholic fatty liver disease--the role of transient elastography and plasma cytokeratin-18
fragments," Alimentary Pharmacology and Therapeutics, vol.
Subsequently, the sections were incubated with mouse anti-rat [alpha]-smooth muscle actin ([alpha]-SMA; 1: 100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), tyrosine kinase receptor (C-kit; 1: 100 dilution, Abcam, UK), cytokeratin-18
(CK-18; 1:50 dilution, Abcam, UK), and matrix metalloproteinase-9 antibodies (MMP-9; 1 : 100 dilution, Abcam) at 4[degrees]C overnight.
Caspases cleave cellular proteins, including cytokeratin-18
To detect DNA fragmentation in epithelial cells in lung tissue M30 (Cytokeratin-18
) and to evaluate apoptosis in kidney tissue active caspase-3 immunohistochemistry was applied to the paraffin sections.
Plasma levels of caspase-generated cytokeratin-18
fragments, a marker of hepatocyte apoptosis, have also been used to identify NASH (non alcoholic steatohepatitis).
The ELISA measures the accumulation of a caspase-cleaved fragment of cytokeratin-18
(CK18-Asp396) in cells and tissue culture medium over time (Kramer et al., 2004).