The cells were incubated overnight with primary antibody (zonula occludens protein 1 [Zo-1] invitrogen, 330100 and cytokeratin 18
Kawakubo et al., "Serum fragmented cytokeratin 18
levels reflect the histologic activity score of nonalcoholic fatty liver disease more accurately than serum alanine aminotransferase levels," Journal of Clinical Gastroenterology, vol.
The sixth paper identified a novel biomarker cytokeratin 18
epitope M30 (M30 CK-18), which was correlated with liver inflammatory activity.
Liu et al., "Cytokeratin 18
knockdown decreases cell migration and increases chemosensitivity in non-small cell lung cancer," Journal of Cancer Research and Clinical Oncology, vol.
The expression of the epithelial cell marker cytokeratin 18
(CK18), were examined in cytoplasm of the IECs by IF, indicating the epithelial characteristic of these cells (Figure 2E).
(CK-18) is a major intermediate filament protein associated with the structural changes characteristic of apoptosis in the liver .
Immunohistochemically, cells stain positive for antibodies to cytokeratin 8 (CAM 5.2), actin, vimentin and cytokeratin 14 and negative for cytokeratin 18
Ohno et al., "Immunohistochemical detection of cytokeratin 18
and its neo-epitope in human salivary glands and pleomorphic adenomas," Anticancer Research, vol.
is an M-cell marker in porcine Peyer's patches.
M30 antibody detects degraded fragments of cytokeratin 18
(dCK18) in the cytoplasm of a cell undergoing apoptosis and is considered the cytoplasmic end product of apoptosis.
Cells were then incubated in blocking solution overnight at 4[degrees]C with primary antibodies as follows: rabbit polyclonal anti-human albumin (ALB) and mouse monoclonal anti-human cytokeratin 18
(CK-18) (all 1:100) (DakoCytomation, Glostrup, Denmark).
Caspase cleaved cytokeratin 18
(CK18) is an early marker of apoptosis.