Immunohistochemically, cells stain positive for antibodies to cytokeratin 8 (CAM 5.2), actin, vimentin and cytokeratin 14
and negative for cytokeratin 18 and 19.
Quantitative real-time PCR was performed using the StepOnePlus equipment (Applied Biosystems) with TaqMan[R] Fast Universal PCR Master Mix (2X), without AmpErase[R] UNG (Applied Biosystems), and predesigned primers for cytokeratin 14
, a-SMA, AQP5, nestin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
For immunohistofluorescence staining, cross sections were hydrated and subjected to antigen retrieval in tris-ethylenediaminetetraacetic acid buffer (cytokeratin 14
, cytokeratin 10, filaggrin, collagen type IV, CD-31, von Willebrand Factor) or citrate buffer (vimentin) for 20 minutes at 100[degrees]C.
The low-grade fibromatosis-like spindle cell carcinoma tumor cells entrap normal breast glandular structures (A), and are highlighted by cytokeratin 14
(B), vimentin (C), and p63 (D) immunohistochemical stains (hematoxylin-eosin, original magnification X20 [A]; original magnification X20 [B through D]).
The following polyclonal primary antibodies were obtained from GeneTex (Irvine, USA): rabbit anti-human cytokeratin 5 (CK-5, Cat #GTX113219), cytokeratin 10 (CK-10, Cat #GTX108883), cytokeratin 14
(CK-14, Cat #GTX104124), loricrin (Cat #GTX116013), and involucrin (Cat #GTX116012); rabbit anti-human transglutaminase-1 (TGM-1) polyclonal antibody (Cat #12912-3-AP) was from Proteintech (USA).
They identified one protein, cytokeratin 14
, or K14, that was present in almost all leader cells but was very rare in the noninvasive parts of the tumour.
In myoepithelioma, tumor cells stain positive for S-100 protein, alpha-SMA, muscle-specific actin, vimentin, pankeratin, cytokeratin 7, cytokeratin 14
, GFAP, EMA, and calponin.
Odontomas express cytokeratin 14
, which is absent in advanced amelogenesis, and cytokeratin 7, which is present in Hertwig root sheath and stellate reticulum.
(9) Cytokeratin 14
(CK14) and cytokeratin 17 (CK17) have both been reported as positive immunostains for BCC, (10) although CK14 has not been studied in BCCm.
Distribution of p63, cytokeratins 5/6 and cytokeratin 14
in 51 normal and 400 neoplastic human tissue samples using TARP-4 multi-tumor tissue microarray.
as a marker of squamous differentiation in transitional cell carcinomas.
Can renal oncocytoma be differentiated from its renalmimics?: the utility of anti-mitochondrial, caveolin-1, CD63 and cytokeratin 14
antibodies in the differential diagnosis.