When SARA was induced, acyl-CoA synthetase (ACS) and cytochrome b5 involved in lipid metabolism were up-regulated, phosphoenolpyruvate carboxykinase (PEPCK) involved in glucose metabolism was downregulated, while enolase was up-regulated.
Cytochrome b5 is known to function as an electron transfer component in a number of oxidative pathways, including the anabolic metabolism of fats and steroids.
The nicotinamide adenine dinucleotide (NADH) pathway, catalyzed by cytochrome b5
reductase, is the most important.
Congenital or inherited causes of methemoglobinemia result due to decreased activity of the enzyme cytochrome b5
reductase resulting in diminished enzymatic reduction of the hemoglobin molecule.
3]) by losing electron is prevented by cytochrome b5
system of the body and normally only 1-2% of hemoglobin is present in ferric state in the body.
reductase deficiency and mental retardation.
Hereditary methemoglobinemia, characterized by a deficiency of NADH-dependent cytochrome b5
reductase, has a wide geographic distribution (2).
The description of the inheritance of congenital cytochrome b5
reductase deficiency as an autosomal recessive condition in the context of the father and two sons being clinically affected is unusual but possible.
The reaction mixture with CYP3A4/OR or CYP3A5/OR was additionally supplemented with purified cytochrome b5
(P450:b5 ratio, 1:1) according to a previously described method (26) before the start of the reaction with NADPH.
reductase activity could not be measured in the neonates because of the lack of technical facilities.
Comparisons of the cytochrome b5
-like domain with cytochrome b5
are shown in Figure 3.
Cyt P450: cytochrome P450, Cyt b5: cytochrome b5
, Cyt P450 R: NADPH- cytochrome P450 reductase, Cyt b5 R: NADH-cytochrome b5
reductase, GST: glutathione S-transferase and DTD: DT-diaphorase.