While preparing the slide from cytocentrifuged
material, it appeared to be of gritty sensation, probably, owing to calcified debris.
For light microscopy analyses, hemolymph samples were cytocentrifuged
(200 X g, 5 min) and hemocytes monolayers were stained with May Griinwald-Giemsa reagent.
The culture suspension was examined microscopically by Wright-Giemsa staining of cytocentrifuged
preparations of cells at 2- to 3-day intervals.
95% alcohol--successful (direct smear ; 3 cytocentrifuged
prep ; not specifically stated ) Air dry--not successful 1 Abbreviations: ASCO, American Society of Clinical Oncologists; CAP, College of American Pathologists.
Tachyzoites can occasionally be seen on cytocentrifuged
cerebrospinal fluid samples stained with Giemsa.
After 5 days of induction, the cells were harvested and cytocentrifuged
onto a microscope slide using a cytospin (Shandon Southern Instrument Inc.
The CSF was cytocentrifuged
(Thermo Scientific Shandon Cytospin 4 Cytocentrifuge, UK) at 1500 rpm for five minutes.
1), (2), (3), (4) CAP's Hematology/Clinical Microscopy Resource Committee suggests all CSF specimens should have a cytocentrifuged
Wright's-stained slide made for analysis, regardless of cell count--always reporting a differential if abnormal cells are identified and always requiring a pathologist review of CSF slides if organisms or malignant cells arc identified.
slides of spun-down lavaged cells were prepared for each animal and stained with Diff-Quik (Dade Behring, Newark, DE, USA) for cell differential counts.
Samples of the hemocyte suspension (50-100 [micro]l) were cytocentrifuged
onto slides with a Shandon Instrument Cytospin II running at 500 rpm for 2 min.
Differential cell counts were made from cytocentrifuged
preparations that were fixed and stained with a Microscopy Hemacolor-Set.
The preparation type included classic preparations (conventional smears and cytocentrifuged
slide preparations), as well as liquid-based preparations (ThinPrep, Hologic, Inc, Bedford, Massachusetts).