For light microscopy analyses, hemolymph samples were cytocentrifuged
(200 X g, 5 min) and hemocytes monolayers were stained with May Griinwald-Giemsa reagent.
The culture suspension was examined microscopically by Wright-Giemsa staining of cytocentrifuged
preparations of cells at 2- to 3-day intervals.
After 5 days of induction, the cells were harvested and cytocentrifuged
onto a microscope slide using a cytospin (Shandon Southern Instrument Inc.
The preparation type included classic preparations (conventional smears and cytocentrifuged
slide preparations), as well as liquid-based preparations (ThinPrep, Hologic, Inc, Bedford, Massachusetts).
The CSF was cytocentrifuged
(Thermo Scientific Shandon Cytospin 4 Cytocentrifuge, UK) at 1500 rpm for five minutes.
1), (2), (3), (4) CAP's Hematology/Clinical Microscopy Resource Committee suggests all CSF specimens should have a cytocentrifuged
Wright's-stained slide made for analysis, regardless of cell count--always reporting a differential if abnormal cells are identified and always requiring a pathologist review of CSF slides if organisms or malignant cells arc identified.
slides of spun-down lavaged cells were prepared for each animal and stained with Diff-Quik (Dade Behring, Newark, DE, USA) for cell differential counts.
Differential cell counts were made from cytocentrifuged
preparations that were fixed and stained with a Microscopy Hemacolor-Set.
False-positive rates by participant type (pathologist versus cytotechnologist) and the general category, reference diagnosis, and preparation type (Papanicolaou-stained smears, modified Giemsa-stained smears, or cytocentrifuged
preparations) were analyzed for the pathologists' responses in order to determine the frequency distribution of the pathologists' responses and the rates of false-positive responses for these lesions according to participant and preparation types.
Body fluids should always be microscopically examined after Gram stain of a cytocentrifuged
The other fraction was cytocentrifuged
Then 150 [micro]L of the suspension was cytocentrifuged
at 150 x g (800 rpm) for 10 minutes, and slides were stained with Gimenez (20).