cycloheximide


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cycloheximide

(sī″klō-hĕks′ă-mīd)
A cytotoxic protein that inhibits the synthesis of proteins by cells.
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References in periodicals archive ?
During 14-day incubation at room temperature, on days 5, 10 and 14 of culture inoculation, the grown colonies were inspected on Sabouraud agar (without cycloheximide).
Caption: Figure 4: The kinetics of degradation of RT1.14opt-in and RT1.14oil in HeLa cells after blocking of translation with cycloheximide. (a) Western blotting of HeLa cells transfected with pVaxRT1.14opt-in and pVaxRT1.14oil plasmids 0, 2, 4, and 6 hours after cycloheximide treatment.
AD: Alzheimer's disease ALS: Amyotrophic lateral sclerosis AO: Antioxidants ARE: Antioxidant response element Cdk5: Cyclin-dependent kinase 5 CHX: Cycloheximide CNS: Central nervous system CQ: Chloroquine ER: Endoplasmic reticulum GFAP: Glial fibrillary acidic protein HD: Huntington's disease IP3: 1,4,5-Trisphosphate type 3 MAM: Mitochondrion-associated ER membrane Nrf2: Nuclear factor erythroid 2-related factor 2 PD: Parkinson's disease ROS: Reactive oxygen species Sig-1R: Sigma-1 receptor.
Abbreviations: CA, carnosic Acid; CDKs, cyclin dependent kinases; CHX, cycloheximide; DAPI, 4',6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; FBS, fetal bovine serum; GBM, glioblastoma; LCC, lactacystin; mAb, monoclonal antibody; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NA, normal astrocytes; ROS, reactive oxygen species; SDS, sodium dodecyl sulfate.
Nonetheless, the effect of luteolin (5 [micro]M) in increasing ALP activity was completely eliminated in the presence of cycloheximide (1 [micro]M) (13) (P = 0.008 vs.
(E) Box plot of [EC.sub.10] values by population for two example chemicals with different potency levels, showing significant population differences by analysis of variance (top; cycloheximide, p = 6.0 x [10.sup.-6]; bottom; triamterene, p = 3.6 x [10.sup.-4]).
The first aim of this study was efficacy and comparison of paraffin baiting technique with other methods such as conventional media including Sabouraud dextrose agar, Sabouraud dextrose agar with cycloheximide, and paraffin agar, to isolate Nocardia from various clinical specimens.
Alternatively, PBMCs were pretreated for 1 hour (37[degrees]C, 5% C[O.sub.2]) with ranolazine (ITK Diagnostics), trimetazidine (Sigma), pertussis toxin (Enzo Life Sciences), etomoxir (Sigma) (inhibitors of [beta]-oxidation, n = 3), aspirin (Aspegic injection powder, n = 3), cycloheximide (Sigma, n = 6 to 7), anti-IL-10 antibody IgG2a (BioLegend, n = 10 to 12), or IgG2a isotype control (BioLegend, n = 10 to 12) prior to stimulation.
Subsequently oocytes were washed three times and cultured in PZM3 with 10 g/ml of cytochalasin B (CB) and 10 g/ml of cycloheximide. Four hours later activated oocytes were washed three times and cultured in PZM3.
Cryopreservation of cattle oocytes: effect of meiotic stages cycloheximide treatment and vitrification procedure.
Under GF conditions, we observed FT induction around the wounded intestinal tissue resulting from a needle puncture, or in the small intestinal epithelial cells of mice intraperitoneally injected with cycloheximide or emetine (i.e., protein synthesis inhibitors).
Cycloheximide: inhibition of Dermocystidium marinum in laboratory stocks of oysters.