CCNB1

(redirected from cyclin B1)

CCNB1

A gene on chromosome 5q12 that encodes cyclin B1, which is essential for controlling the cell cycle at the G2/M transition. Cyclin B1 interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex, the maturation promoting factor, and interacts with RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
References in periodicals archive ?
Thus, the aim of this study was to investigate the effects of GA on apoptosis and necrosis of non-tumorigenic mice TM4 Sertoli cells via detecting the mitochondrial dysfunction, Cyclin B1, proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (BAX), and Caspase-3 gene expression.
In this table, unstained cells were indicated by a 0, slightly stained by 1, moderately stained by 2 and intensively stained cells were indicated by the number 3 Cyclin B1 1.
Approximately 75 [micro]g of lysate was resolved on 12% SDS-PAGE, electrotransferred to PVDF membranes (Dingguo, China), and then incubated with specific primary rabbit polyclonal antibodies to cyclin B1, p27, and caspase-3 at 4[degrees]C overnight.
MiR-30a-5p overexpression inhibits breast cancer cell proliferation by downregulating UBE3C, as well as cyclin B1, cyclin D1, and c-myc [14].
It also arrested cycling cells at S and [G.sub.2]/M phases and affected cyclin B1, Bcl-2, and Ki67 expression.
The following primary antibodies were used in this study: [alpha]-SMA, [alpha]1(I) procollagen, and fibronectin (Epitomics, San Francisco, CA, USA); TGF-[beta]RI, TGF-[beta]RII, PDGF-[beta]R, EGF-R, and Bcl-2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); cyclin A, cyclin B1, CDK1, CDK2, Bax, pro-caspase-9, cleaved-caspase-9, pro-caspase-8, cleaved-caspase-8, pro-caspase-7, cleaved-caspase-7, pro-caspase-3, cleaved-caspase-3, full-length PARP-1, cleaved-PARP-1, and [beta]-Actin (Cell Signaling Technology, Danvers, MA, USA).
Moreover, the maintenance of M phase protein cyclin B1 is required for G2 phase arresting and megakaryocyte endomitosis.
CA inhibits CDKs activity via modulation of both Cyclin B1 and [P21.sup.WAF] expression and causes a G2/M block in GBM cells
Symbol Gene name 1 CCNA1 Cyclin A1 2 CCNA2 Cyclin A2 3 CCNB1 Cyclin B1 4 CCND1 Cyclin D1 5 CCND2 Cyclin D2 6 CCND3 Cyclin D3 7 CCNE1 Cyclin E1 8 CCNE2 Cyclin E2 9 CCNH Cyclin H 10 CDC25A Cell division cycle 25 homolog A (S.
Our QPCR results have revealed also a notable upregulation of expression of cyclin B1 and Cdk1 mRNA.
These molecules included p53, MUC-1, heat shock proteins (HSP-27, HSP-60, and HSP90), HER2/neu/c-erg B2, GIPC-1, c-myc, c-myb, cancertestis antigens (NY-ESO-1), BRCA1, BRCA2, endostatin, lipophilin B, cyclin B1, cyclin D1, fibulin, insulin-like growth factor binding protein 2 (IGFBP-2), topoisomerase II alpha (TOPO2[alpha]), and cathepsin D (for review see [9,15]).
Apart from its anti-inflammatory activity, GLT suppressed cell proliferation of RAW264.7 cells through cell cycle arrest at G0/G1-G2M, which was mediated by the down-regulation of expression of cell cycle regulatory proteins cyclin D1, CDK4 and cyclin B1, respectively.