Cyclerion Therapeutics is a clinical-stage biopharmaceutical company harnessing the power of soluble guanylate cyclase
(sGC) pharmacology to discover, develop and commercialize breakthrough treatments for serious and orphan diseases.
(b) Methylene blue's site of action, as a guanylyl cyclase
inhibitor, resulting in increased vasomotor tone.
After filtering with Multiscreen Assay System filtration plates (Millipore, Billerica, MA, USA), 0.1 ml cADPR or the samples and 50 [micro]l of a reagent containing 30 mmol/L nicotinamide, 0.3 [micro]g/ml ADP-ribosyl cyclase
, and 100 mmol/L sodium phosphate, pH 8.0, were mixed and incubated for 15 min at room temperature.
Specific primers for adenylyl cyclase
subtypes and the calcium-related protein RyR3 target genes are shown in Table 1.
During acquisition, 50 [micro]M Forskolin (adenylate cyclase
activator) is added at 5 minutes of time and 10 [micro]M Rolipram+ 500 [micro]M IBMX (phosphodiesterase inhibitors) were added at time point of 15 minutes.
Dahl et al., "Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate cyclase
in pituitary cells," Biochemical and Biophysical Research Communications, vol.
(QC), an enzyme found in animals and plants, catalyzes the cyclization of N-terminal Glutamine, and to a lesser extent Glutamate, to pyroglutamate (1).
Pituitary adenylate cyclase
activating polypeptide (PACAP) has neuroprotective effects in different neuronal and retinal injuries.
Uroguanylin (UGN) is an endogenous peptide that acts on membrane-bound guanylate cyclase
receptors of intestinal and renal cells increasing cGMP production and regulating electrolyte and water epithelial transport.
[beta] Adrenergic receptor--G protein (Gs)--adenylate cyclase
The non-selective nitric oxide synthase inhibitor L-NAME, as well as the soluble guanylate cyclase
inhibitor MB, attenuated the relaxation of EOFAZ in endothelium-intact rat thoracic aortic rings.
We have used [alpha] and [beta]-adrenoceptor agonists and antagonists for modulation of the activity of [G.sub.s]- and [G.sub.i] proteins of the membrane adenylate cyclase