Cyclerion Therapeutics is a clinical-stage biopharmaceutical company harnessing the power of soluble guanylate
cyclase (sGC) pharmacology to discover, develop and commercialize breakthrough treatments for serious and orphan diseases.
(b) Methylene blue's site of action, as a guanylyl
cyclase inhibitor, resulting in increased vasomotor tone.
After filtering with Multiscreen Assay System filtration plates (Millipore, Billerica, MA, USA), 0.1 ml cADPR or the samples and 50 [micro]l of a reagent containing 30 mmol/L nicotinamide, 0.3 [micro]g/ml ADP-ribosyl
cyclase, and 100 mmol/L sodium phosphate, pH 8.0, were mixed and incubated for 15 min at room temperature.
Specific primers for adenylyl
cyclase subtypes and the calcium-related protein RyR3 target genes are shown in Table 1.
During acquisition, 50 [micro]M Forskolin (adenylate
cyclase activator) is added at 5 minutes of time and 10 [micro]M Rolipram+ 500 [micro]M IBMX (phosphodiesterase inhibitors) were added at time point of 15 minutes.
Dahl et al., "Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate
cyclase in pituitary cells," Biochemical and Biophysical Research Communications, vol.
Glutaminyl
cyclase (QC), an enzyme found in animals and plants, catalyzes the cyclization of N-terminal Glutamine, and to a lesser extent Glutamate, to pyroglutamate (1).
Pituitary adenylate
cyclase activating polypeptide (PACAP) has neuroprotective effects in different neuronal and retinal injuries.
Uroguanylin (UGN) is an endogenous peptide that acts on membrane-bound guanylate
cyclase receptors of intestinal and renal cells increasing cGMP production and regulating electrolyte and water epithelial transport.
[beta] Adrenergic receptor--G protein (Gs)--adenylate
cyclase system.
The non-selective nitric oxide synthase inhibitor L-NAME, as well as the soluble guanylate
cyclase inhibitor MB, attenuated the relaxation of EOFAZ in endothelium-intact rat thoracic aortic rings.
We have used [alpha] and [beta]-adrenoceptor agonists and antagonists for modulation of the activity of [G.sub.s]- and [G.sub.i] proteins of the membrane adenylate
cyclase system.
