Hemoglobin concentration (g/dL) was estimated using hemoglobin kit according to cyanomethemoglobin
method and the packed cell volume (%) by microhematocrite centrifuge.
Hemoglobin was measured in triplicate via spectrophotometry (Milton Roy, Spectronic 20D) by use of a cyanomethemoglobin
solution (Sigma Chemical # 525-A) immediately after it was drawn.
After entering the body, cyanide seeps into the circulatory system and forms a complex with methemoglobin, resulting in cyanomethemoglobin
Y) at a wave length of 540 nm was used to determine the hemoglobin values by using a cyanomethemoglobin
standard, respectively .
Hemoglobin estimation by cyanomethemoglobin
Deoxygenated/oxygenated hemoglobin, under the influence of specific chemical agents, is changed into derivatives: methemoglobin, S-nitrosohemoglobin, carbonous oxide hemoglobin, sulphhemoglobin, cyanomethemoglobin
5, equivalent to that of cyanomethemoglobin
and a molecular weight of 16,500 for myoglobin was assumed.
Hemoglobin levels were determined by using the cyanomethemoglobin
method (HemoCue, Cypress, CA, USA).
Cyanide antidote kit additionally contains sodium thiosulfate which converts cyanomethemoglobin
to the less toxic thiocyanate (1).
The blood specimens were assayed for: number of erythrocytes (RBC), counted in a Neubauer chamber; hematocrit (Ht) by the microhematocrit technique; and hemoglobin level (Hb) by the cyanomethemoglobin
Packed Cell Volume (PCV) and Hemoglobin estimation (Hb) were determined by microhaematocrit and cyanomethemoglobin
methods, respectively (Ghai, 1999).
1-3) Accurate measurement of the Hgb concentration in avian blood by the traditional cyanomethemoglobin
technique requires lysis of erythrocytes followed by centrifugation to remove free nuclei.