cyanomethemoglobin

cyanomethemoglobin

[sī′ənō′met·hē′məglō′bin]
Etymology: Gk, kyanos + meta, together with, haima, blood; L, globus, ball
product of an in vitro reaction in which hemoglobin from whole blood is reduced to methemoglobin and converted to a stable red pigment using potassium ferricyanide and potassium cyanide. Cyanmethemoglobin has absorption peaks at 416 and 541 nm.
References in periodicals archive ?
Hemoglobin concentration (g/dL) was estimated using hemoglobin kit according to cyanomethemoglobin method and the packed cell volume (%) by microhematocrite centrifuge.
Hemoglobin was measured in triplicate via spectrophotometry (Milton Roy, Spectronic 20D) by use of a cyanomethemoglobin solution (Sigma Chemical # 525-A) immediately after it was drawn.
After entering the body, cyanide seeps into the circulatory system and forms a complex with methemoglobin, resulting in cyanomethemoglobin.
Y) at a wave length of 540 nm was used to determine the hemoglobin values by using a cyanomethemoglobin standard, respectively [13].
Hemoglobin estimation by cyanomethemoglobin method.
Deoxygenated/oxygenated hemoglobin, under the influence of specific chemical agents, is changed into derivatives: methemoglobin, S-nitrosohemoglobin, carbonous oxide hemoglobin, sulphhemoglobin, cyanomethemoglobin [3].
5, equivalent to that of cyanomethemoglobin and a molecular weight of 16,500 for myoglobin was assumed.
Hemoglobin levels were determined by using the cyanomethemoglobin method (HemoCue, Cypress, CA, USA).
Cyanide antidote kit additionally contains sodium thiosulfate which converts cyanomethemoglobin to the less toxic thiocyanate (1).
The blood specimens were assayed for: number of erythrocytes (RBC), counted in a Neubauer chamber; hematocrit (Ht) by the microhematocrit technique; and hemoglobin level (Hb) by the cyanomethemoglobin method.
Packed Cell Volume (PCV) and Hemoglobin estimation (Hb) were determined by microhaematocrit and cyanomethemoglobin methods, respectively (Ghai, 1999).
1-3) Accurate measurement of the Hgb concentration in avian blood by the traditional cyanomethemoglobin technique requires lysis of erythrocytes followed by centrifugation to remove free nuclei.