cyanogen bromide


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cyanogen bromide

reagent used to fragment peptide chains, since it cleaves only those peptide bonds in which methionine contributes the carbonyl group. Also used as coupling agent in affinity chromatography where it reacts with the Sepharose matrix forming a reactive product to which proteins, nucleic acids or other biopolymers can be coupled.
References in periodicals archive ?
The weight loss of caprine native pericardium cross-linked with GA and DPPA for 12 h and treated with cyanogen bromide for 12 h exhibited lesser degree of weight loss in comparison to that observed in 72 h cross-linked tissues.
Cyanogen bromide cleaves proteins at methionine residues of collagen whereas, elastin lacks methionine, therefore, it is resistant to cyanogen bromide digestion.
For this purpose, a safe method of cyanogen bromide preparation was introduced [54].
Preparing method of cyanogen bromide for activation of agarose.
The biomaterials cross-linked with EGDGE and DPPA did not showed any appreciable resistance to cyanogen bromide, pepsin and papain degradation.