The hemoglobin concentration was determined using a colorimetric kit (Randox), based on the
cyanmethemoglobin method.
Hemoglobin (Hb) was detected by
cyanmethemoglobin method, Packed Cell Volume (PCV) by microhematocrit (Apel, HC 702, Japan), and Total Erythrocyte Count (TEC) and Total Leukocyte Count (TLC) by Neubauer's haemocytometer.
Plasma samples were sent to the laboratory and analyzed for complete blood count (erythrocytes, hemoglobin, and hematocrit); mean corpuscular hemoglobin concentration (MCHC), by the
cyanmethemoglobin colorimetric technique; mean corpuscular volume (MCV), by the micro-hematocrit method; and leukogram (leukocytes, segmented neutrophils, red blood cells, lymphocytes, and neutrophils), by the MayGrunwald Giemsa method.
Hemoglobin was estimated by
cyanmethemoglobin method by commercially available Drabkin's reagent solution (Sigma-Aldrich Co USA.), ferritin by radioimmunoassay kit (Beckman Coulter USA).
The hemoglobin concentration in the hemolysates was determined by the standard
cyanmethemoglobin method.
Measurement of haematological parameters: Haemoglobin in blood was measured by the standardised
cyanmethemoglobin method.
In the complete blood count, the erythrocyte count (RBC) was determined in a Neubauer chamber, while the hematocrit (Ht) was determined through the microhematocrit method and the hemoglobin concentration (Hb) through the
cyanmethemoglobin method.
The
cyanmethemoglobin method was used to measure Hb.
Red and white blood cell count was determined with chamber method using Neubauer's haemocytometer; haemoglobin concentration with
cyanmethemoglobin method [13] and haematocrit in capillary tubes of 75 [micro]L volume, which were centrifuged in a microhematocrit centrifuge and the haematocrit values were read with a reader.
Hemoglobin (Hb) was measured using
cyanmethemoglobin method (Jain, 1986).
The haematology analytes were assayed on the ADVIA[R] 2120i System with Autoslide using wet chemistry, flow cytometry, and peroxidase staining methodologies, with the exception of the haemoglobin assay that uses a standard
cyanmethemoglobin colorimetric method [34].
Hemoglobin concentration in hemolysates was determined by standard
cyanmethemoglobin method.
