Two ml blood sample was collected from each patient in EDTA vials and haemoglobin levels were estimated by cyanmethaemoglobin
method in institute's haematology labaratory.
Haemoglobin concentration was determined by a cyanmethaemoglobin
method using Drabkin's solution as diluent .
Hb values are measured by Coulter(r) LH 780 Hematology Analyzer (Beckman Coulter, Miami, United States) and cyanmethaemoglobin
method in our hospital.
To measure iron deficiency anemia, hemoglobin levels were estimated for the children by the gold standard Cyanmethaemoglobin
Hemoglobin (Hb) was estimated by cyanmethaemoglobin
method and TEC and DLC estimation was done as per Coles (1980).
Hb was measured by modification of the cyanmethaemoglobin
method together with a cell-based method.
The HemoCue Hb meter used in our study is reliable compared with direct cyanmethaemoglobin
in venous blood, allowing for the slightly lower red cell volume in capillary blood (1-3% lower) (Sari et al, 2001).
5.0ml of cyanmethaemoglobin
was dispensed into a test tube.
Estimation of Hemoglobin: Hemoglobin concentration was estimated using colorimetric procedure by Cyanmethaemoglobin
The hemoglobin (Hb) concentration in hemolysate was determined by the cyanmethaemoglobin
Studies in India have shown that as compared to classical cyanmethaemoglobin
method, Haemocue overestimates Hb levels (9-13).
Hb concentration was determined with the cyanmethaemoglobin