cumulus cells

cumulus cells

those cells which surround and nurture an egg cell.
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2009), and miRNAs demonstrate spatio-temporal expression in oocytes, granulosa cells (GCs), cumulus cells, and preimplantation embryos (Abd El Naby et al.
GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or [alpha]-minimum essential medium ([alpha]-MEM).
Cumulus-oocyte complexes (COCs) that were surrounded by a minimum of three cumulus cells were selected for culture.
After culturing for 38 h to 42 h, oocytes with expanded cumulus cells were briefly treated with 0.
Therefore, BMP15 coordinates with FSH to promote the development of cumulus cells and maintain their competence to undergo expansion and steroidogenesis (30).
Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP-15 and GDF9 control cholesterol biosynthesis in cumulus cells.
The signaling needed for the completion of meiosis is a kind of chemical conversation between the egg called an oocyte and cells that surround it, called cumulus cells.
Then, the cumulus cells were removed by vortexing and oocytes were fixed in 4% paraformaldehyde during 15 min and transferred to 0.
The observation that segmentation occurred more often with cumulusfree oocytes suggests that cumulus cells produce a component(s) that delays the degenerative process.
To avoid disruption of the surrounding cumulus cells, an 18-gauge needle was used.
Medium used should be made as closely as possible to the conditions of oocyte maturation in vivo so as to keep the gap junctions between oocytes and cumulus cells that surround it.
Ten to fifteen selected oocytes with a homogenous and evenly granulated cytoplasm and three to more layers of cumulus cells were washed four times in maturation medium and placed into 50 ul droplet of maturation medium with or without GTE under paraffin oil.