They first harvested and cultured keratinocytes
from biopsies of the patients' unwounded, unscarred skin and transduced these samples with COL7A1-containing retrovirus, producing eight gene-corrected grafts for each patient.
Grund et al., "Organization and formation of the tight junction system in human epidermis and cultured keratinocytes
," European Journal of Cell Biology, vol.
They achieved this by using contact sensitizers in cultured keratinocytes
derived from the bulge of plucked haired follicles also known as bulge-derived keratinocytes (BDKs) .
mRNA was obtained from cultured keratinocytes
at 1, 4, 24, 48 and 72h of culture in the presence and absence of 1uM RA.
Transcriptional profiling of epidermal keratinocytes: comparison of genes expressed in skin, cultured keratinocytes
, and reconstituted epidermis, using large DNA microarrays.
Recently, we demonstrated that PRGF induces the antimicrobial peptide hBD-2 in primary keratinocytes  showing for the first time that PRGF is able to induce the expression of antimicrobial peptides in cultured keratinocytes
as well as in wounded skin.
PITX2 overexpression activates P21 expression in cultured keratinocytes
"There is lots of research going on," including stem cell therapy leading to fibroblast differentiation, grafting cultured keratinocytes
onto skin, and intravenous collagen, she said.
This hypothesis was validated by in vitro testing in the cultured keratinocytes
: SIRT1 overexpression inhibited proliferation rate, whereas reducing SIRT1 increased keratinocyte proliferation.
UVB activates ERK1/2 and p38 signaling pathways via reactive oxygen species in cultured keratinocytes
. J Invest Dermatol 112:751-756 (1999).
Ferreira, "Gene expression profile of cytokines and receptors of inflammation from cultured keratinocytes
of burned patients," Burns, vol.
The engineered skin substitutes were composed of autologous cultured keratinocytes
and dermal fibroblasts attached to a collagen-based, biopolymer scaffold, Dr.