Retinal radial serial 10 [micro]m thick sections were obtained by cryotome
(Kryostat 1720, Leitz Wetzlar).
Specimens were then frozen and coronally sectioned in 20 mm sections in a cryotome
at -20[degrees]C (Hyrax C52, Zeiss) in Tissue-Tek O.C.T.
The procedure of freezing and cutting the frozen tissue block in a cryotome
and subsequently staining it for histopathologic examination in the frozen section suite can take up to 20 to 30 minutes.
After gamma counting, partially necrotic muscle tissues were cut into sections of 10 [micro]m using a cryostat microtome (Shandon Cryotome
FSE; Thermo Fisher Scientific Co., MA) and thaw-mounted on glass slides.
The spinal cords were postfixed and then cryoprotected gradually up to 30% sucrose in PBS, embedded in OCT, frozen on dry ice, and sectioned in a cryotome
The brain in the OCT block was cut into 15 [micro]m sections using a cryostat (Thermo-Shandon Cryotome
E; Thermo Electron Corporation, Waltham, MA).
We designed the present morphological study in rats by combining intravascular injections of multifunctional contrast materials, "real-time" digital radiography (DR) for microangiogram , histomorphology of cryotome
and paraffin sections with special and standard stains, and mathematical assumptions in an attempt to experimentally elucidate the pathways of LPD trafficking through the hepatic microcirculation.
Thick sections (15 [micro]m) were cut on a cryotome
and processed for immunofluorescence staining.
Specimens for frozen section were frozen to -22 [degrees]C within 10 minutes in the Shandon Cryotome
SME Cryostat (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 5-micron-thick sections of the surgical margin were stained for 3 minutes with hematoxylin-eosin (H&E) stain.
Frozen sections were cut with a Shandon Cryotome
SME (Thermo Electron Corporation, Pittsburg, PA, USA) and placed on poly-L-lysine-coated slide.
After 7, 14, and 56 days, tissue samples containing the PLA meshes were collected from 8 randomly selected animals, and cryosections (5 [micro]m) were prepared with a Cryotome
2800 Frigocut N (Reichert-Jung, Nu[beta]loch, Germany).
Para el corte histologico de los encefalos se utilizo el criostato de congelacion Thermo Shandon Cryotome
. Para la colecta de los registros se utilizo el programa Scope[R] version 3.7 (ADInstruments), mientras que para la representacion grafica de los resultados se utilizo Origi version 5.0 (Microcal Software Inc.).