Spermatic suspension was mixed with cryoprotective
solution (1:3) into cryotubes to be equilibrated for 5 min at 5[degrees]C.
Fahy says that with a mixture of cryoprotective
agents he can minimize the toxicity, maximize the material's glass-forming ability and balance the cryoprotectant levels inside and outside the cells.
Duman, "An osmotin-like cryoprotective
protein from bittersweet nightshade Solanum dulcamara," Plant Molecular Biology, vol.
effect of trolox demonstrated with three types of human cells.
Purification, immunolocalization, cryoprotective
, and antifreeze activity of PCA60: a dehydrin from peach (Prunus persica).
For motilities, a significant interaction was noted between the cryoprotective
treatment and the chamber type, whereby motilities for DMSO and GLY were higher (P = 0.0055) using MCC.
It maintains integrity of the skin under freezing conditions showing high cryoprotective
Although they constitute an important source of energy in overwintering plants, their depolymerization at subfreezing temperatures is thought to be an essential source of cryoprotective
sugars (Olien and Clark, 1993; Livingston, 1996).
With his group's method of systematically separating components of blood by size and weight and then testing their cryoprotective
properties, Loomis hopes to identify new cryoprotectants in other freeze-tolerant animals such as a tidal marsh snail.
All antioxidants contributed to a cryoprotective
effect, suppressing lipid peroxidation in the sperm membranes.
Wu et al., "Synthesis of Clinked AFGP analogues and assessment of their cryoprotective
properties," Bioconjugate Chemistry, vol.