Cryopreservation bags are used to store red cells with cryoprotectant
One extracellular CPA used in research involving testicular cryopreservation is sucrose, which is always associated with intracellular CPAs in cryoprotectant
addition protocols (BAERT et al.
Vitrification is a physical process in high concentrated cryoprotectants
without formation of any ice crystals.
Explants survival percentage (a) and percentage of shoot formation (b) from lateral buds encapsulated in sodium alginate matrix after 30 days in culture, after incubation in PVS2 cryoprotectant
solution for different times (0, 15, 30, 60 and 120 minutes), before immersion in LN.
are any substance, either biological or chemical, which protects the microbial or eukaryotic cells/tissues/organ from degradation or inactivation during freeze-storage.
Radecka, Bacillus subtilis Natto: a Non-Toxic Source of Poly-g-glutamic Acid that Could be Used as a Cryoprotectant
for Probiotic Bacteria.
sup] To our knowledge, L-proline has not been used as a cryoprotectant
for mammalian oocytes.
This study aimed at evaluating the effect of ACP[R]-104 as a cryoprotectant
solution for cryopreservation of dourado sperm on the fertilization rate, comparing these results with those obtained when using the cryoprotectant
solution that has been recommended for the species (Carolsfeld et al.
8 to 8), with a chelating agent (say, EDTA) with an added cryoprotectant
such as glycerol or DMSO, is probably the safest matrix in which to store a nucleic acid which will be frozen and thawed.
For the definition of cryoconservation protocols becomes necessary to know several factors: sperm characteristics of the species, extender composition, sperm:extender ratio, cryoprotectant
and dosage, cooling rate and thawing, equilibrium time and fertilization tests.
Thus, the success of cryopreservation is linked with the water balance of the cells and the use of cryoprotectant
substances (VOLK; WALTERS, 2006).
The effects of overrun and glycerol as a cryoprotectant
on viability of probiotics in frozen yogurt were investigated.