FFB of the parietal pleural was taken with flexible FB-35C-1 Olympus forceps with cusps being 2.4 mm for the outer diameter and a length of 3.5 mm; the CB sample was obtained using a flexible cryoprobe
with a 1.9 mm diameter and a 900 mm length (20416-037, Erbokryo CA, Erbe, Germany) and the CPB sample was sampled via Abram's needle (Figure 1).
Local anesthesia was given by injection of 2-5 mL of 2% lidocaine proximal to the tumor lesion(s) and along the course of the cryoprobes
. One or more cryoprobes
(1.47-mm diameter 17-gauge ISOTHERM IceRod, IceRod Plus, and IceSphere needles; Galil Medical) were inserted into the targeted tumor using CT guidance, through a small skin incision of 1-2 mm.
All [sup.13]C-[sup.1]H gradient heteronuclear single quantum coherence (gHSQC) NMR experiments were performed using an Agilent Inova 750 MHz spectrometer equipped with an H/C/N 5 mm-PEG triple resonance cryoprobe
Cryosurgery is a technique to treat tumor and can be used inside the body and on the skin using extreme cold which is obtained using liquid nitrogen through cryoprobe
into or on the target region.
Aggressive use of lens loop, phacoprobe, cryoprobe
or vigorous irrigation of vitreous cavity is avoided.
After opening the pericardium, a prechilled cryoprobe
(AC3: Brymill Cryogenics with a 3 mm flat surface cryoprobe
) was placed against the epicardial surface of the heart for 5 seconds.
After this company was sold in 2005, Dr Altman became a partner of CryoProbe
, a cryogenic probe technology company.
Proton (1H-NMR) and carbon ([sup.13]C-NMR) spectroscopy analysis were performed by using Spectrometer FT-NMR 600 MHz CryoProbe
, Advance 111 600 MHz model.
Finally, the electronically chilled cryoprobe
is used to rapidly chill the area being treated.
(7,8) In 1977, Rodgers and colleagues demonstrated that the endobronchial application of a cryoprobe
to tracheal stenoses in sheep was not only safe but that the lesions healed with minimal submucosal fibrosis?
All [sup.1]H NMR spectra were obtained on a Bruker DRX-600 Avance NMR spectrometer operating at 600.13 MHz for [sup.1]H resonance frequency using an inverse detection 5 mm [sup.1]H-[sup.13]C-[sup.15]N cryoprobe
attached to a CryoPlatform (the preamplifier cooling unit).