[sup],,,,, Furthermore, the incidence of congenital anomalies was not increased in live born babies from oocyte cryopreservation, when compared with natural conception.
Thus, immature oocyte cryopreservation seemed to be a safer option for women to reserve the fertility.
[sup] reported that human oocytes matured in vitro after cryopreservation at the GV stage showed an increased incidence of chromosomal and spindle abnormalities, as well as lower maturation rates.
There are two cryopreservation methods: slow-freezing and vitrification.
[sup],,,,, Vitrification is superior to slow-freezing in human embryo cryopreservation.
Assessments of DNA integrity, spindle configuration, aneuploidy and genomic imprinting after cryopreservation
In molluscs, larvae prior to the formation of the larval shell (D-larvae) are typically selected as experimental material in cryopreservation studies (Naidenko 1997, Chao et al.
virginica Paniagua-Chavez and Tiersch (2001) reported that the postthaw survival rate of trochophore larvae (12 h postfertilization at 25[degrees]C) was affected by the larval concentration used during cryopreservation, and decreased as the larval concentration increased, from about 100% at 10 individuals [mL.sup.-1] to less than 40% at 100 individuals [mL.sup.-1] and further decreased to less 10% when larval concentration increased to 10,000 individuals [mL.sup.-1].
However, the results from this study highlight the potential to develop a practical larval cryopreservation technique(s) in this species.
Cryopreservation of spermatozoa of the Japanese oyster, Crassostrea gigas.
Cryopreservation of early larvae and embryos in oyster and hard claro.
Cryopreservation of finfish and shellfish gametes and embryos.