The process of forming a cryoprecipitate from solution.
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IGF-I was released from binding proteins by acid-ethanol extraction, followed by neutralization and cryoprecipitation (19).
Cryoglobulinemia was assessed using tube and gel-diffusion cryoprecipitation (18) (OKAZAKI et al., 1998).
Sample processing - To prevent cryoprecipitation of VWF and other proteins, blood samples for VWF assays should be transported to the laboratory at room temperature.
(2) The processed serum is observed for cryoprecipitation by daily inspection at 4[degrees]C for seven days.
Cryoglobulin was investigated using both tube and gel cryoprecipitation. (6) Smooth muscle IgG antibodies were tested by indirect fluorescent antibody test using antigenic substrates of rodent rat liver-kidney-stomach sections and sera diluted at 1:40.
The IGF-I in plasma was extracted with acid-ethanol cryoprecipitation and analyzed using specific radioimmunoassay kits consisting of anti-hIGF-I, [sup.125]I-IGF-I and hIGF-I (Diagnostic System Laboratories, USA).
It is essential that fasting serum samples be collected at 37[degrees]C to avoid false positives due to cryoprecipitation of lipoproteins or false negatives due to premature cryoprecipitation of immunoglobulins, rheumatoid factor, and complement (3).
Others are made of a variety of materials designed to selectively retain specific plasma components by cryoprecipitation (removal of cryoglobulins) or affinity adsorption (such as removal of IgG-class antibodies by adsorption to Staphylococcus protein A).
These cases remind us that IgM monoclonal proteins (M-proteins) have earned a reputation as saboteurs of many laboratory assays owing to their self-aggregation, aggregation of latex particles, binding to analytes and reagents, and cryoprecipitation. One additional point is that some IgM M-proteins also play havoc with their own detection and measurement by electrophoretic techniques.
Cryoglobulins that precipitate significantly at 20 to 30 C and as high as 36 C have been described(2) and may be associated with more serious symptomatology than other cryoglobulins with a lower cryoprecipitation point, present in significantly higher concentrations.(1)