IGF-I was released from binding proteins by acid-ethanol extraction, followed by neutralization and cryoprecipitation
Fresh frozen plasma, cryoprecipitation
and prothrombin complex were given to correct coagulopathy.
Cryoglobulinemia was assessed using tube and gel-diffusion cryoprecipitation
(18) (OKAZAKI et al.
Sample processing - To prevent cryoprecipitation
of VWF and other proteins, blood samples for VWF assays should be transported to the laboratory at room temperature.
Before assay, IGF-I was extracted with acid-ethanol cryoprecipitation
(Breier et al.
2) The processed serum is observed for cryoprecipitation
by daily inspection at 4[degrees]C for seven days.
The design of the CryoSeal FS System is based upon two of the Company's recent technological breakthroughs enabling real time processing of blood products, including: (a) automated cryoprecipitation
technology which prepares fibrinogen-rich cryoprecipitate from plasma in as little as 51 minutes, as compared to two to three days for standard blood center methodology; and (b) thrombin activation technology which prepares activated human thrombin from a small aliquot of the same human plasma.
Cryoglobulin was investigated using both tube and gel cryoprecipitation
The IGF-I in plasma was extracted with acid-ethanol cryoprecipitation
and analyzed using specific radioimmunoassay kits consisting of anti-hIGF-I, [sup.
It is essential that fasting serum samples be collected at 37[degrees]C to avoid false positives due to cryoprecipitation
of lipoproteins or false negatives due to premature cryoprecipitation
of immunoglobulins, rheumatoid factor, and complement (3).
Others are made of a variety of materials designed to selectively retain specific plasma components by cryoprecipitation
(removal of cryoglobulins) or affinity adsorption (such as removal of IgG-class antibodies by adsorption to Staphylococcus protein A).
These cases remind us that IgM monoclonal proteins (M-proteins) have earned a reputation as saboteurs of many laboratory assays owing to their self-aggregation, aggregation of latex particles, binding to analytes and reagents, and cryoprecipitation