electron cryomicroscopy

(redirected from cryo-EM)

electron cryomicroscopy

,

cryo-EM

Electron microscopic imaging of rapidly frozen molecules and crystals in solution.
References in periodicals archive ?
The special electron microscope, called Cryo-EM, freezes objects to almost -300 degrees Fahrenheit and then takes an image of them.
But labs are now racing to adopt the cryo-EM method, because it can take pictures of proteins that can't easily be formed into large crystals.
Our Krios has become the leader in cryo-EM for structural biologists seeking to gain new insights into protein structure and function that will lead, ultimately, to a better understanding of the causes of disease and the development of new drugs and vaccines, " said Peter Fruhstorfer, vice president and general manager, life sciences, Thermo Fisher Scientific.
In a second instance of EM technology playing an important role, I spoke with two researchers from the Medical Research Council (UK) who recently used cryo-EM to reveal, for the first time, the atomic structure of tau filaments, the protein linked to a variety of neurodegenerative diseases including Alzheimer's and CTE.
Heptares Therapeutics, the wholly-owned subsidiary of Sosei Group Corporation has partnered with four pharmaceutical companies (Astex Pharmaceuticals, AstraZeneca, GlaxoSmithKline and UCB); the UK Medical research Council's Laboratory of Molecular Biology (MRC-LMB); the Nanoscience Centre of the University of Cambridge; and FEI Company, the manufacturer of high performance microscopes, to form the Cambridge-Pharmaceutical Cryo-EM Consortium, the first of its kind worldwide.
Thanks to a number of innovative hardware and software advances, cryo-EM has undergone a "resolution revolution" in image quality over the past several years.
After many trials, we successfully developed a cryo-EM, and then improved the resolution as well as the operational complications of the prototype cryo-EM and have now developed a 7th generation cryo-EM, which is equipped with a top-entry tilting specimen stage.
Cryo-EM is likely to reveal much more about bacterial "bones," features that were invisible with traditional EM.
Frederickson commented that "some of the systems now that are using the cryo-EM [cryoelectron microscopy] are actually getting quite good at characterizing the structure of even relatively large proteins and other types of molecular complexes.
The Titan Krios will be located in the state-of-the-art NUS Cryo-EM facility, led by Prof.
With cryo-EM, you can look at a material that's fragile and chemically unstable and you can preserve its pristine state - what it looks like in a real battery AaAaAeAe-- and look at it under high resolution.
In fact, this year the company announced its 7th-generation cryo-EM (page 12).