They performed cryo-electron microscopy (cryo-EM
) of the drug-virus complex to determine how the drug exerted its effect.
In the past, cryo-electron microscopy, or cryo-EM
, was used for DNA research.
Megabodies are novel antigen-binding chimeric proteins which are ideally suited for three-dimensional structural analysis of proteins via high-resolution cryo-electron microscopy (cryo-EM
The team determined cryo-EM
structures of the substrate-engaged proteasome in seven distinct conformational states, designated as EA1, EA2, EB, EC1, EC2, ED1 and ED2, to nominal resolutions of 2.8-3.6 A.
To crack the telomerase code, Collins and her team used a state-of-the-art cryoelectron microscope (Cryo-EM
) to see the enzyme in action at unprecedented resolutions of seven or eight angstroms.
The special electron microscope, called Cryo-EM
, freezes objects to almost -300 degrees Fahrenheit and then takes an image of them.
Some of the approaches discussed include those that investigate inhibition of the CA enzymatic activity like esterase activity assays and stop flow kinetics and biophysical methods such as thermal shift assays, surface plasmon resonance, mass inlet mass spectroscopy (MIMS), X-ray crystallography, Cryo-EM
, neutron crystallography, and nuclear magnetic resonance (NMR) (Figure 1).
Jacques Dubochet, Joachim Frank and Richard Henderson were awarded the prize for their work in developing cryo-electron microscopy, or cryo-EM
, a technique that fires beams of electrons at proteins that have been frozen in solution to deduce the biomolecules' structure.
"Our Krios has become the leader in cryo-EM
for structural biologists seeking to gain new insights into protein structure and function that will lead, ultimately, to a better understanding of the causes of disease and the development of new drugs and vaccines, " said Peter Fruhstorfer, vice president and general manager, life sciences, Thermo Fisher Scientific.
Even though the overall architecture of the EAG1 channels is similar to that of previously crystallized Kv channel structures, there are many different aspects in S2-S3 linker, S4, and S4-S5 linker based on the structural models of rat EAG1 (rEAG1) channels derived by single-particle cryoelectron microscopy (cryo-EM
Structural studies using cryoelectron microscopy (cryo-EM
) offer methods for examination of molecules/protein complexes in near-native conditions as no crystal needs to be formed [10-13].