After six hours of application of croton oil, the MPO enzyme activity was assessed in the ear samples.
A single topical application of croton oil on the ear induced a marked increase in the ear thickness, with [E.sub.max] of 142 [+ or -] 8 [micro]m when evaluated 6 h after the induction of inflammation process.
Effect of Palmatine on DMBA and Croton Oil Induced Skin Carcinogenesis.
on the other hand, DMBA and croton oil treated animals these layers start differentiating in the form of papilloma with signs of abnormal architecture of the epidermal layer and this was due to irregular proliferation of stratum spinosum cells, with abnormal thickening of the stratum corneum and stratum spinosum (Figure 4(b)).
Mice were treated then with 20 [micro]l of a fresh solution of croton oil (2.5% v/v in acetone) on the inner surface of their right ear.
Ear edema induced by topical croton oil: The topical treatment with FA (20 [micro]l, 100 mg/ml) did not significantly reduce the auricular edema induced by topical croton oil (70.7 [+ or -] 13.4 mg).
Topical application of croton oil
induces an acute inflammatory response mainly characterized by fluid accumulation and edema formation.
Inflammation was induced on the inner surface (about 1 [cm.sup.2]) of the right ear applying 80 [micro]g of Croton oil dissolved in 42% aqueous ethanol (for extracts and the relevant controls) or acetone (for fractions, compounds and their controls).
multifida ethanol extract after 6 h induction of Croton oil mouse ear dermatitis Substance Dose ([micro]g [cm.sup.-2]) No.
Arachidonic acid, croton oil
, and PMA were used as inflammatory agents as previously described (Perez-Garcia et al., 1996).
Groups & Treatment Body weight (mg) Initial Final Only vehicle + DMBA + Croton oil
. 30.0[+ or -]1.64 32.11[+ or -]1.11 Basil leaf extract 31.06[+ or -]1.16 34.22[+ or -]1.22 (150 mg/kg body wt) + DMBA + Croton oil
Cutaneous inflammation was induced on the inner surface of the right ear (surface: about 1 [cm.sup.2]) of anaesthetised mice by application of 80 [micro]g of croton oil
dissolved in an appropriate vehicle, as reported below.