two-dimensional immunoelectrophoresis

(redirected from crossed immunoelectrophoresis)

two-·di·men·sion·al im·mu·no·e·lec·tro·pho·re·sis

a combination of conventional electrophoretic separation and electroimmunodiffusion; electrophoresis is first carried out, then the electrophoretic strip is placed on a second slide and an antibody-containing agarose solution is allowed to solidify adjacent to it; electrophoresis is then performed at right angles to the original separation.
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References in periodicals archive ?
'Cross-reactivity of major outer membrane proteins of Enterobacteriaceae, studied by crossed immunoelectrophoresis', Journal of Bacteriology, 1980; 143(1), 328-337.
Characterization of the [DELTA]K58-[[beta].sub.2]-microglobulin molecule presumably could lead to design specifications for devices to remove it: Does its different mobility on crossed immunoelectrophoresis imply a need to use charged-membrane hemodialysis, which improves removal of glycated [[beta].sub.2]-microglobulin as demonstrated by Randoux et al.
(28,60) Other specialized methods of platelet evaluation, such as crossed immunoelectrophoresis, will be discussed in the following section with the individual disorders for which they are useful.
(40) This finding is virtually diagnostic of Glanzmann thrombasthenia, but the disorder can be confirmed by platelet flow cytometry or crossed immunoelectrophoresis of platelet membrane proteins (Table 2).
These findings were confirmed by crossed immunoelectrophoresis (CIE) (Figure 3).
Signals attributable to proteins cross-reactive with the antibody could be excluded because the high specificity of the anti-SHBG antibody had been verified by crossed immunoelectrophoresis by the manufacturer.
At present, pre-[beta]1-HDL concentrations are measured by various methods, including crossed immunoelectrophoresis (10), two-dimensional electrophoresis (6,11), and sandwich enzyme immunoassays (12).
Another method that he developed for separating and identifying proteins, called crossed immunoelectrophoresis, led to major progress in elucidating the role of proteolytic enzymes and the regulation of their activity.
The specific reaction against human LZ was ascertained using crossed immunoelectrophoresis, which revealed that the LZ precipitation arc appears only against concentrated urine from patients with monocytic leukemia and that no precipitation is observed against nondiseased human plasma or concentrated nondiseased human urine.
As mentioned by its manufacturer, the specificity of the anti-LZ As used in this immunoassay was ascertained using crossed immunoelectrophoresis against concentrated urine from patients with monocytic leukemia, concentrated nondiseased urine, and nondiseased human plasma.