cross hybridization

cross hy·brid·i·za·tion

annealing of a DNA probe to an imperfectly matching DNA molecule.
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Through microarray has the advantage of high flux, it is susceptible to the interference of cross hybridization of homologous miRNA sequences, and the equipment are expensive.
Research Progress of MicroRNA in Early Detection of Ovarian Cancer
Metaphase cells are used to localize the probe and determine its analytical sensitivity and specificity; male cells are used to rule out cross hybridization of the probe with the Y chromosome.
Validation of fluorescence in situ hybridization using an analyte-specific reagent for detection of abnormalities involving the mixed lineage leukemia gene
PrimerPlex creates a pool of primers for each sequence under uniform reaction conditions, checks them for cross hybridization with other primer sets and homologies with the rest of sequences in the pool.
PREMIER Biosoft International Releases PrimerPlex 2.50 with Support for Designing Multiplex PCR Assays

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