Through microarray has the advantage of high flux, it is susceptible to the interference of
cross hybridization of homologous miRNA sequences, and the equipment are expensive.
Metaphase cells are used to localize the probe and determine its analytical sensitivity and specificity; male cells are used to rule out
cross hybridization of the probe with the Y chromosome.
PrimerPlex creates a pool of primers for each sequence under uniform reaction conditions, checks them for
cross hybridization with other primer sets and homologies with the rest of sequences in the pool.