Through microarray has the advantage of high flux, it is susceptible to the interference of cross hybridization
of homologous miRNA sequences, and the equipment are expensive.
Metaphase cells are used to localize the probe and determine its analytical sensitivity and specificity; male cells are used to rule out cross hybridization
of the probe with the Y chromosome.
PrimerPlex creates a pool of primers for each sequence under uniform reaction conditions, checks them for cross hybridization
with other primer sets and homologies with the rest of sequences in the pool.