A brilliant cresyl blue (BCB) staining test can show different intracellular activity of G6PDH in oocytes because the G6PDH enzyme converts BCB from blue to a colorless state.
Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brillant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity.
Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer.
Assessment of porcine oocytes using brilliant cresyl blue.
Developmental competence of porcine oocytes selected by brilliant cresyl blue and matured individually in a chemically defined culture medium.
Supplementation with cysteamine during maturation and embryo culture on embryo development of prepubertal goat oocytes selected by the brilliant
cresyl blue test.
We observed numerous RBCs containing hemoglobin H (HbH) inclusions in the peripheral blood smear stained with brilliant
cresyl blue (Figure1).
Preparation of smear for reticulocyte counts: Three ml of blood was mixed with equal volume of solution made of brilliant
cresyl blue in normal saline and incubated at 37[degrees]C for 15 rain and smears were made.
05 would indicate - -/-[alpha] and not -[alpha]/-[alpha] if brilliant
cresyl blue staining reveals an increase in Hb H inclusion bodies.
max], nm) in solvents obtained with different dyes Solvent Sudan Janus Brillant Black Green
Cresyl Blue (Cl salt) (Cl salt) Acetonitrile 592 651 629 Acetone 600 645 640 Propylene carbonate 605 655 635 Water 635 615 636 Benzene 576 579 607 Methanol 592 653 630 [C.
In vitro generation of Hb H inclusions is accomplished by staining unfixed cells with an oxidative dye such as New Methylene Blue or Brilliant Cresyl Blue.
The Hb H preparation typically is strongly positive in the afflicted fetus, with inclusions representing precipitated Hb Barts, whereas parental blood films stained with Brilliant Cresyl Blue would be expected to show only rare H-containing cells.
