cresol red


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Related to cresol red: phenol red, Thymol blue

cre·sol red

(krē'sol red),
An acid-base indicator with a pK value of 8.3; yellow at pH values below 7.4, red above 9.
Farlex Partner Medical Dictionary © Farlex 2012

cre·sol red

(kre'sōl red)
An acid-base indicator with a pK value of 8.3; yellow at pH values below 7.4, red above 9.0.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

cresol red

An indicator of pH. It is yellow below pH 7.4 and red above 9.0.
See also: red
Medical Dictionary, © 2009 Farlex and Partners
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Treatments were 10% Nu-Lure plus 0.1% cresol red or glass-distilled deionized water plus 0.1% fluorescein or 0.2 M sucrose plus 0.1% fluorescein, or 0.2 M sucrose in 10% Nu-Lure plus 0.1% fluorescein.
All primers were cycled with 2.5 mM Mg[Cl.sub.2], 0.8 mM dNTPs, 0.04 [micro]M primers, 0.25 units of Taq DNA polymerase (Promega, Madison, WI), 20-40 ng of DNA, and cresol red loading buffer (final concentration 2% sucrose and 0.005% cresol red) for 35-45 cycles of 94[degrees]C for 45 seconds, 55[degrees]C for 45 seconds, and 72[degrees]C for 1 minute.
PCR was performed by rapid-cycling techniques [3, 41 in a reaction volume of 10 [micro]L with each dNTP at 200 [micro]mol/L, each primer at 0.5 [micro]mol/L, 3.0 mmol/L Mg[Cl.sub.2], 50 mmol/L Tris, pH 8.3, 500 mg/L bovine serum albumin, 20 g/L sucrose, 0.1 mmol/L cresol red, 50 ng of DNA, and 0.4 U of Taq polymerase.