creatinase

creatinase

 [kre-at´ĭ-nās]
an enzyme that catalyzes the decomposition of creatine into urea and ammonia.

cre·a·ti·nase

(krē'ă-tĭ-nās),
An enzyme catalyzing the hydrolysis of creatine to sarcosine and urea.

cre·a·ti·nase

(krē-at'i-nās)
An enzyme catalyzing the hydrolysis of creatine to sarcosine and urea.
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References in periodicals archive ?
We used an enzymatic method to measure creatinine, based on the conversion of creatinine with the aid of creatininase, creatinase, and sarcosine oxidase to glycine, formaldehyde, and hydrogen peroxide.
Creatinase [creatine amidinohydrolase (EC 3.5.3.3)] converts creatine to sarcosine and urea (1-3).
One of these enzymes is creatinase, an inducible enzyme expressed in bacteria when creatinine is provided as the main source of carbon or nitrogen (17-22).
Creatinine: This enzymatic method is based on the conversion of creatinine with the aid of creatininase, creatinase, and sarcosine oxidase to glycine, formaldehyde, and hydrogen peroxide.
Contract awarded for Ern laboratory - march biochemicals (eci psa, phenytoin, amylase, ck creatinase, ckmb, ggt, lactate, lipase, protein csf, transferrin, eci beta, etc
Urine creatinine was measured by the modified kinetic Jaffe method in 1988-2006 and by an enzymatic (creatinase) method in 2007-2008 (NCHS 2010a).
Urine creatinine concentrations were measured using Creatinine Plus Assay, which is a coupled enzymatic reaction using creatininase, creatinase, and sarcosine oxidase (Roche Diagnostic, Indianapolis, Ind).
Creatine is then converted to sarcosine and urea via creatinase. Sarcosine oxidase converts sarcosine to glycine and hydrogen peroxide, and the hydrogen peroxide reacts with a chromophore HTIB (2,4,6-triiodo-3-hydroxybenzoic acid) by the use of peroxidase to produce a quinone imine chromogen product measured at 546 nm (secondary wavelength at 700 nm).
This method is based on the same reaction sequence as the Creatinine-PAP (phenol aminophenazone) method (29) consisting of 4 consecutive enzymatic steps via creatininase, creatinase, sarcosine oxidase, and peroxidase, but using a different chromophore (2,4,6-triiodo-3-hydroxybenzoic acid) and a modified detergent composition.
The enzymatic method is based on the determination of sarcosine after conversion of creatinine with creatininase, creatinase, and sarcosine oxidase.
Know the laboratory analytical methods (e.g., Jaffe vs creatinase) for the assessment of renal function (creatinine, urea nitrogen, glomerular filtration rate) and proteinuria.
One method was based on coupling of enzymatic bioassays using creatininase, creatinase, and sarcosine oxidase and electrophoresic separation and amperometric detection of reaction products (46).