To ensure cell viability was not compromised, we cultured ECs on gelatin-coated and SLIPS-treated glass coverslips
were mounted onto glass slide using 50% glycerinum.
fornicata, while the other 25 individuals were allowed to attach to glass coverslips
, and then also sewn into individuals mesh bags.
After being washed with PBS, coverslips
were incubated with 100 [micro]l of Click-iT reaction cocktail for 30 min.
We used n=1 (one coverslip
containing around 5-6 expressing cells).
Mix a large drop of honey with a drop of water on a microscope slide and apply a coverslip
The cells were seeded in Petri dishes containing coverslips
with stretched pieces of VMHE-f or VMHE-m at densities: for 3T3 cells  [2.
The cells on the coverslips
were rinsed, dehydrated, and mounted on a coverslip
using the aqueous PVA-DABCO antifading mounting medium (Sigma-Aldrich).
When not in use, store the hemacytometer and coverslips
in a container to help keep them free from dust and scratches.
1]) was placed on glass coverslips
(22 x 22 mm) and incubated in a moist chamber.
" were prepared by cutting plastic slide coverslips
(from Fisher Scientific) into the appropriate sizes with a razor blade.
For investigation of fluorescence images (by use of confocal microscopy), cells were plated on glass coverslips
and grown to 90% confluence, after which they were incubated with Ln[P0.