For [.sup.3]H-thymidine labelling index analysis (which determines cells in the S phase), HeLa cells were seeded into round coverslips
which were in 24-well plates at a density of 2x104 cells per well and incubated for 24 hrs.
Freshly isolated neutrophils were cultured with enriched iRBCs onto coverslips
, and their phagocytic activity was examined at different time points after staining with Giemsa.
MSCs were seeded on coverslips
and PLGA scaffolds in a 24-well culture plate in n-MEM supplemented with 10% FBS (HyClone).
To ensure cell viability was not compromised, we cultured ECs on gelatin-coated and SLIPS-treated glass coverslips
. By live cell staining and quantification, we confirmed that the SLIPS treatment did not compromise cell viability as the untreated and SLIPS-treated groups revealed no difference in the fluorescence values by staining with calcein AM (Figure 1D).
3T3 fibroblast cells were seeded at 6 x [10.sup.4] cells/well in 6-well microplates containing coverslips
(13 mm diameter and 0.13 mm thickness) for sterile microscopy.
Sample preparation was carried out by dipping PBS solution containing cells into a glass chamber, consisting of two sandwiched coverslips
glued by two parafilm strips also acting as spacers.
fornicata, while the other 25 individuals were allowed to attach to glass coverslips
, and then also sewn into individuals mesh bags.
[sup] Briefly, RT4 cells were seeded onto a 60-mm dish containing a coverslip
. After treatment, cells were fixed with 4% paraformaldehyde in PBS followed by permeabilization with 0.25% Triton X-100.
We used n=1 (one coverslip
containing around 5-6 expressing cells).
We introduce a new method of floating plastic microscope slide coverslips
as the substrate for Sommerstorffia attachment.
6.6 Wash an equal number of microscope coverslips
, or device substrates, serially in acetone, methanol, and deionized water, and blow-dry with nitrogen.
Warner Instruments' Quick Release Chambers are designed to quickly form coverslip-bottom imaging chambers using standard size coverslips
. Features include silicone O-ring seals; magnetic clamping; and use of standard size coverslips