Cortical neurons were dispersed with a neuronal medium (Sciencell) with 1% (vol/vol) neuronal growth supplement (Sciencell), and then they were seeded at a density of 5 x [10.sup.5]/ml onto coverslips
precoated with poly-L-lysine in 6-well plates (Corning).
with three individual surfaces (plasma-exposed, gelatin-coated and SLIPS-treated) were prepared and kept in a 24-well plate.
In the present SEM analyses, large numbers of cells with normal morphology and cytoplasmic projections on coverslip
surfaces were observed in the presence of SB, SBU, and AMU adhesives.
The sample was prepared by pipetting 50 f L of a water suspension of mature PY79 wild-type spores at a concentration of 105 spores/mL on a clean microscope glass coverslip
and left to dry at ambient conditions.
Thirteen of the juveniles (mean shell length, 3.75 mm; SD, 0.19 mm) were then allowed to attach individually to glass coverslips
(22 X 22 mm).
RT4 cells were seeded on 8 mm round coverslips
in DMEM medium.
microvascular endothelial cells (PMVECs) were seeded on 35mm round coverslips
in 6-well dish plates and incubated for 24 hours (cells attained 70-80% confluence).
Device bonding involves exposing the surfaces of the hard silicone and glass coverslip
, or other silica substrate, to an oxygen plasma to clean and chemically activate the surfaces.
The culture medium and medicine were carefully aspirated without touching the coverslip
. The cells were then washed twice with sterile PBS solution for 2 minutes each time.
Hypoxia was induced using the coverslip
hypoxia model  with some modifications.
Mix a large drop of honey with a drop of water on a microscope slide and apply a coverslip
. Or mix a large drop of honey with a drop of Lugol's solution and apply a coverslip
This test is usually called the "coverslip
test." Test articles are placed in a Petri dish where the surface is inoculated and covered with a glass coverslip