Cover slip was placed and observed under phase contrast microscope.
To check the initial infection,
cover slips were removed from some wells and air dried immediately (at 1 hour).
Although the height of the peaks are similar on the untreated
cover slip (25 nm) and the
cover slip treated with 5N NaF (23 nm), there is variation in the number of peaks (7 x [10.sup.3] on untreated, and 4.2 x [10.sup.3] on 5N etched surface).
After rinsing and air- drying
cover slips were mounted with Balsam of Canada and two hundred spermatozoa were evaluated in each smear with phase contrast microscope at 1000x.
Biofilms were grown on borosilicate
cover slips in 50 ml Falcon tubes as described earlier.
The
cover slips (22 mm x 22 mm) were warmed on the heating plate.
The glass
cover slips were weighed before and after loading the catalysts already prepared in colloidal dispersions to find out the amount of catalyst deposited on pieces of
cover slips.
Level of abundance was roughly estimated to distinguish organisms that were "abundant" (4 or more specimens/coverslip) or "common" (2-3 specimens/per coverslip) from those that were "uncommon" (5-15 specimens/I5
cover slips) or "rare" (fewer than 5 specimens/15
cover slips).
The clinician transfers the serous fluid to a glass slide, either by direct application of the slide to the lesion, or by transferring the fluid with a bacteriologic loop or the edge of a
cover slip. If necessary to prevent drying of the specimen, a drop of non-bacteriostatic normal saline may be placed on the slide; however, the saline might dilute the specimen and reduce test sensitivity.
A micropipette was used to place about 250 mg of wet diet onto a glass
cover slip (22 x 22 mm).
In order study the morphological characteristics with reference to aerial mycelium, substrate mycelium and sporulation, the isolated colonies were cultivated on glycerol asparagines agar by simple "Inclined
cover slip culture technique" (Gibbs & shapton, 1968) as detailed below.
Further grinding reduced the specimen to the final thickness and a
cover slip was then placed on the slide.