was placed and observed under phase contrast microscope.
To check the initial infection, cover slips
were removed from some wells and air dried immediately (at 1 hour).
Although the height of the peaks are similar on the untreated cover slip
(25 nm) and the cover slip
treated with 5N NaF (23 nm), there is variation in the number of peaks (7 x [10.sup.3] on untreated, and 4.2 x [10.sup.3] on 5N etched surface).
After rinsing and air- drying cover slips
were mounted with Balsam of Canada and two hundred spermatozoa were evaluated in each smear with phase contrast microscope at 1000x.
Biofilms were grown on borosilicate cover slips
in 50 ml Falcon tubes as described earlier.
The cover slips
(22 mm x 22 mm) were warmed on the heating plate.
The glass cover slips
were weighed before and after loading the catalysts already prepared in colloidal dispersions to find out the amount of catalyst deposited on pieces of cover slips
Level of abundance was roughly estimated to distinguish organisms that were "abundant" (4 or more specimens/coverslip) or "common" (2-3 specimens/per coverslip) from those that were "uncommon" (5-15 specimens/I5 cover slips
) or "rare" (fewer than 5 specimens/15 cover slips
The clinician transfers the serous fluid to a glass slide, either by direct application of the slide to the lesion, or by transferring the fluid with a bacteriologic loop or the edge of a cover slip
. If necessary to prevent drying of the specimen, a drop of non-bacteriostatic normal saline may be placed on the slide; however, the saline might dilute the specimen and reduce test sensitivity.
A micropipette was used to place about 250 mg of wet diet onto a glass cover slip
(22 x 22 mm).
In order study the morphological characteristics with reference to aerial mycelium, substrate mycelium and sporulation, the isolated colonies were cultivated on glycerol asparagines agar by simple "Inclined cover slip
culture technique" (Gibbs & shapton, 1968) as detailed below.
Further grinding reduced the specimen to the final thickness and a cover slip
was then placed on the slide.