counterstain

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counterstain

 [kown´ter-stān]
a stain applied to render the effects of another stain more discernible.

count·er·stain

(kown'ter-stān),
A second stain of different color having affinity for tissues, cells, or parts of cells other than those taking the primary stain used to render more distinct the parts taking the first stain.

counterstain

(koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. counter·stained, counter·staining, counter·stains
To color (a microscopic specimen) with such a stain.

count·er·stain

(kown'tĕr-stān)
A second stain of a different color, having affinity for tissues, cells, or parts of cells other than those taking the primary stain, used to render more distinct the parts taking the first stain.
Synonym(s): contrase stain.
References in periodicals archive ?
(b) Anti-CD68 immunohistochemistry with USPIO particles counterstaining shows several brown stained macrophages (white arrow heads) and blue iron particles within the structures (black arrows) which are consistent with positively stained vessels on anti-CD31 staining (magnification x200).
Panel (a) overlay of HBcAg (red) and EpCAM (green) positive cells and DAP1 nuclei counterstaining (blue) in HCC tissue (panels (a1)-(a3), resp.).
The denaturation and hybridization of the probe and the target DNA with recommended parameters of time and temperature, final post-hybridization washes and counterstaining with 4'6-diamidino 2-phenylindole hydrochloride (DAPI) were carried out as per manufacturer's protocol on the patient slide.
After washing, images were visualized using 3,3'-diaminobendizine (DAB), with hematoxylin counterstaining for 1 min.
Counterstaining with hematoxylin-eosin was performed for regular histologic characterization of the tumor.
TUNEL-positive nuclei were counted in ten nonoverlapping fields per coverslip and then converted to percentage by comparing TUNEL-positive counts with the total cell nuclei as determined by DAPI (4',6'-diamino-2-phenylindole) counterstaining.
Counterstaining with hematoxylin was performed for nuclear staining.
Immunoreactions were detected and visualized with the polymer-peroxidasemethod (EnVision+/HRP and 3,3-diaminobenzidine; Dako Cytomation, Denmark) followed by counterstaining with Mayer hematoxylin.
All steps were performed on Discovery XT Automated Immunostainer using VMSI validated reagents, including deparaffinization, cell conditioning (antigen retrieval with a borate-EDTA buffer), primary antibody staining, detection and amplification using a biotinylated-streptavidin-HRP and DAB (Diaminobenzidine) system, and hematoxylin counterstaining. Following staining on the instrument, slides were dehydrated through graded alcohols to xylene and coverslipped with mounting medium.