counterstain

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counterstain

 [kown´ter-stān]
a stain applied to render the effects of another stain more discernible.

count·er·stain

(kown'ter-stān),
A second stain of different color having affinity for tissues, cells, or parts of cells other than those taking the primary stain used to render more distinct the parts taking the first stain.

counterstain

/coun·ter·stain/ (-stān) a stain applied to render the effects of another stain more discernible.

counterstain

(koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. counter·stained, counter·staining, counter·stains
To color (a microscopic specimen) with such a stain.

counterstain

a second stain added to a previously stained tissue sample to make cellular details more distinct.

count·er·stain

(kown'tĕr-stān)
A second stain of a different color, having affinity for tissues, cells, or parts of cells other than those taking the primary stain, used to render more distinct the parts taking the first stain.
Synonym(s): contrase stain.

counterstain

a stain applied to render the effects of another stain more discernible.
References in periodicals archive ?
The pathologist should also note the pattern of malignant cells (glands versus diffuse, sheets of tumor cells versus interspersed benign inflammatory/stromal cells) and the shape and relative size of the malignant cell nuclei (round versus oval, medium versus large) to assist scorers in identifying those malignant cells after hybridization and counterstaining.
In future work, differences in phagocytic activities of eosinophilic and basophilic granulocytes in 3 bivalve species can be carried out by counterstaining of neutral red and a fluorescent tracer, such as fluorescent bead.
Chromogenic ISH assays have several advantages: 1) the assay is interpreted via a standard light microscope; 2) the photo-stability of chromogenic ISH signals enable re-interpretation after long-term storage; and 3) hematoxylin counterstaining creates excellent tissue morphology and improved ability to correlate ISH signals with specific tissue areas.
After three washes in PBS and counterstaining with DAPI (4', 6'-diamidino-2-phenylindole), the cells were mounted in glycerol/PBS solution (1:1).
Visualization was done with diaminobenziding tetrachloride and counterstaining was performed with haematoxylin.
Binding was detected by using monoclonal antibody 2G3 against RHDV, biotinylated anti-mouse IgG, horseradish peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole substrate with hemalum counterstaining, as described (2).
Briefly, after removal of paraffin and rehydration of the tissue sections, enzyme retrieval was done with1% trypsin in PBS for 20minutes at 37oC, inactivation of endogenous Peroxidase done by 3% hydrogen peroxide, incubation with primary polyclonal antibodies for 60 minutes in humidifier chamber, incubation with Biotinylated anti-rabbit antibody for 30 min, peroxidase-conjugated streptavidin and incubated for 10-15 min and finally counterstaining was done with a bath of Mayer's hematoxylin for 2-3 min and sections were examined under microscope.
After counterstaining with hematoxylin, the slides were dehydrated with grade series of alcohol, cleared in xylene, and mounted with a coverslip.
mairei was hybridized in situ to denatured metaphase chromosomes, hybridized Festuca chromosomes fluoresced green, while Lolium chromosomes fluoresced red as a result of counterstaining with propidium iodide.