counterstain

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counterstain

 [kown´ter-stān]
a stain applied to render the effects of another stain more discernible.

count·er·stain

(kown'ter-stān),
A second stain of different color having affinity for tissues, cells, or parts of cells other than those taking the primary stain used to render more distinct the parts taking the first stain.

counterstain

(koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. counter·stained, counter·staining, counter·stains
To color (a microscopic specimen) with such a stain.

count·er·stain

(kown'tĕr-stān)
A second stain of a different color, having affinity for tissues, cells, or parts of cells other than those taking the primary stain, used to render more distinct the parts taking the first stain.
Synonym(s): contrase stain.
References in periodicals archive ?
PPAR[gamma] activity was first assayed by immunofluorescence: (A) cells were fixed, stained with phalloidin (red), and counterstained with DAPI (blue); then (B) PPRE-H2B-eGFP fluorescence intensity was quantified.
Nuclei (in blue) were counterstained with haematoxylin.
Immunofluorescence colocalization of CD31 (red) and vimentin (green) counterstained with DAPI (blue) was carried out in indicated groups (scale bars: 200 [micro]m).
Ultrathin sections were counterstained with uranyl acetate and lead citrate and examined with a transmission electron microscope.
SK-4100), counterstained with hematoxylin, dehydrated, cleared, and mounted.
Some of the tissue sections from the heart, lungs, liver, and spleen were stained with alum hematoxylin (Harris hematoxylin) and counterstained with 90% ethanolic extract of Z.
Darkly stained organisms (contrasting with paler or pink counterstained background of keratinocytes) were evident on microscopic examination with mature filaments of D.
The immunoreactions were then visualized with a 3,3-diaminobenzidine solution (Beijing Zhongshan Biotechnology Co., Ltd, Beijing, China), counterstained with hematoxylin, and mounted.
Subsequently, the sections were washed in tap water for 10 min, counterstained with Mayer's hematoxylin, washed in tap water, and dehydrated in graded ethanol.