counterstain


Also found in: Dictionary, Thesaurus, Encyclopedia, Wikipedia.

counterstain

 [kown´ter-stān]
a stain applied to render the effects of another stain more discernible.

count·er·stain

(kown'ter-stān),
A second stain of different color having affinity for tissues, cells, or parts of cells other than those taking the primary stain used to render more distinct the parts taking the first stain.

counterstain

(koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. counter·stained, counter·staining, counter·stains
To color (a microscopic specimen) with such a stain.

count·er·stain

(kown'tĕr-stān)
A second stain of a different color, having affinity for tissues, cells, or parts of cells other than those taking the primary stain, used to render more distinct the parts taking the first stain.
Synonym(s): contrase stain.
References in periodicals archive ?
Cells were washed in PBS and counterstained with Hoechst 33342 nucleic acid stain for 10 minutes.
Some of the tissue sections from the heart, lungs, liver, and spleen were stained with alum hematoxylin (Harris hematoxylin) and counterstained with 90% ethanolic extract of Z.
A total of 10-15 metaphase spreads were analyzed, using a fluorescence microscope (AxioImager.Z1 mot, Zeiss) equipped with appropriate filter sets to discriminate between a maximum of five fluorochromes and the counterstain DAPI (Diaminophenylindol).
Briefly, conjunctival scrape smears were covered with 30 microliters of Evans blue counterstain containing solution of fluorescein-isothiocyanate(FITC-) conjugated antibodies for 20min at 20[degrees]C in a dark, humidified chamber.
Slides were washed with PBS and mounted in Vectashield Mounting Medium (Vector Labs) containing the dye 4,6-diamido-2-phenylindole (DAPI) to counterstain nuclei.
The analysis of antibody binding was performed with a diaminobenzidine (DAB) kit, and hematoxylin was used as the counterstain. The slices were dehydrated through graded ethanol and xylene and mounted with Entellan.
Counterstain Hematoxylin was applied for 2 minutes and washed with distilled water.
DAB was used as the chromogen, and Mayer hematoxylin was used as the counterstain. The positive control was normal testis tissue, which shows cytoplasmic staining in Leydig cells.
Acid fuchsin counterstain (5 mL of 1% acid fuchsin + 95 mL of picric acid + 0.25 mL of 12 N hydrochloric acid) was added to the cell layers for 5 minutes.
The slides were developed in 3,37-diaminobenzidine (DAB), followed by a haematoxylin counterstain. Sections from nonneoplastic colonic mucosa from non-FAP patients, normal breast, and endometrium were used as positive controls, and for each case a section from which the primary antibody was replaced by phosphate buffered saline was used as a negative control.