CA and FA were minor components representing up to 6.5% and 4.5%, respectively (Table 1), whereas coumaric
acid could not be detected by HPLC-DAD.
The HPLC elution profile recorded at 280 nm showed 5 phenolic acids: gallic acid, epicatechin, coumaric
acid, apigenin, and naringenin (Figure 1(a)) with concentrations of 1111.41 [micro]g/mL, 198.52 [micro]g/mL, 147.73 [micro]g/mL, 7.89 [micro]g/mL, and 6.13 [micro]g/mL, respectively (Table 2), while the HPLC elution profile of flavonoids (Figure 1(b); Table 2) recorded at 360 nm identified 3 compounds: rutin, quercetin, and kaempferol, with concentrations of 50.74 [micro]g/mL, 6.78 [micro]g/mL, and 14.42 [micro]g/mL.
The p-hydroxybenzoic and vanillic acids, quercetin and epicatechin were also detected in high levels in all organs (especially in leaves), along with luteolin (higher in flowers), gallic and coumaric
acids (higher in roots), coumaric
being the only phenolic present in all extracts.
Regarding the content of the other three cinnamic acid derivatives (coumaric
, ferulic, and rosmarinic acids), an increase in the germinated extracts of L.
Caffeic (Sigma-Aldrich) coumaric
(Sigma-Aldrich), ferulic (Merck) and chlorogenic (Sigma-Aldrich) acids were used as standards at 1mg*[ml.sup.-1].
(22) This component of the varietal aroma of Gewurztraminer is likely due to an unusually high quantity of ferulic and coumaric
acids present in the juice of this variety.
(3) 1 unit = 1 [micro]mole coumaric
acid accumulated [h.sup.-1] [mg.sup.-1] protein.
In addition, p-hydroxybenzoic (2), chlorogenic (3), vanillic (4), caffeic (5), and o- coumaric
acids (10) were also present in comparatively lower quantities (Fig.
"Garlic contains lots of phytochemicals, such as allicin, saponin and coumaric
acid," adds Zied.
The salicylic acid, formic acid, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), trolox (6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid), fluorescein sodium, flavonoids (mangiferin, rutin, isoquercitrin, quercetin, kaempferol, naringin, naringenin, linarin, luteolin, acacetin, and apigenin), and phenolic acids (shikimic, protocatechuic, 4-hydroxybenzoic, vanillic, syringic, quinic, chlorogenic, caffeic, coumaric
, synaptic, ferulic, and 4,5-dicaffeoylquinic acids) were all obtained from Sigma Aldrich (St.
DPPH (2,2-diphenyl-l-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid), Folin-Ciocalteu (FC) reagent, capsaicin, dihydrocapsaicin myricetin, catechin, resveratrol, gallic acid, coumaric
acid, caffeic acid, ferulic acid, sinapic acid, and chlorogenic acid were purchased from Sigma-Aldrich (St.
oxysporumdid produce chemicals materials as organic acids, especially phenolic acids such as cinnamic, vanillic, coumaric
, and ferulic acid .