cotransfection

cotransfection

/co·trans·fec·tion/ (ko″trans-fek´shun) simultaneous transfection with two separate, unrelated nucleic acid molecules, one of which may contain a gene that is easily assayed and acts as a marker.
References in periodicals archive ?
42,43) Cotransfection of mPGES-2 with either COX isozyme demonstrated that mPGES-2 could be coupled with both COX-1 and COX-2, leading to [PGE.
Recombinant HIV-1 HXBc2 CAT virus was produced by cotransfection of 293T cells with the pSVC21 [Vpr.
Cotransfection of 3 or 4 plasmids was conducted: actual lentiviral construction encoding a viral RNA genome; one (pCMV[DELTA]R8.
Cotransfection of TALENs and ssODNs into primary fibroblasts enabled efficient isolation of MSTN-modified colonies.
The vectors were packaged into the viral particles by cotransfection with MoMuLV gag-pol and VSVG-encoding plasmids into 293T cells.
Then the recombinant adenovirus (Ad-KLF-4) was rescued via the cotransfection with the adenoviral genomic plasmid and the shuttle plasmid.
Cotransfection of Huh7 cells with expression constructs encoding HCV core, E1, E2, NS4B, or NS5A and an ARE-dependent luciferase reporter construct indicates that selective overproduction of these proteins is able to trigger activation of Nrf2.
In cardiomyocytes transfected with pGL2(-642) GDF15, cotransfection of the FSTL1 expression plasmid resulted in a 5.
For example, upon cotransfection of HEK293 cells with both the BK channel main a-subunit and an auxiliary [beta]1-subunit, the resulting BK channel is composed of [alpha]-[beta]1 complexes.
Retroviral particles pseudotyped with SARS-CoV S were made by cotransfection of an S-expressing plasmid, pCAGGS-S, with plasmids encoding MLV or HIV gag-pol and GFP vector genome in 293T cells.
Finally, a stable cell line will be generated from cotransfection of this QC expression vector and a selection vector, pCoHYGRO.
Overexpression of catalytically dead mutants for PHDs, cotransfection of siRNAs for PHDs and substitution of Pro3 (94) with an alanine residue (P394A) elevated the constitutive activity of TRPA1 and abolished its responses to hypoxia, but not those to hyperoxia.