cosmid


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cos·mid

(koz'mid),
A recombinantly engineered plasmid, a circular DNA containing, in order: a plasmid origin of replication and a drug-resistance marker, the cos (cohesive end) site from bacteriophage λ, and a fragment of eukaryotic DNA to be cloned; cosmids are constructed to permit cloning of fragments of up to about 40,000 base pairs in length, with one or more unique restriction sites being necessary to facilitate cloning.

cosmid

(kŏz′mĭd)
n. Genetics
An artificially constructed plasmid used for cloning large genes or other DNA sequences.

cosmid

a class of CLONING VECTOR which comprises a bacterial PLASMID and the COS SITE of a BACTERIOPHAGE, typically bacteriophage lambda. A cosmid combines features of plasmids and of bacteriophages and can be used in CLONING. Large DNA fragments, up to 40 KILOBASE pairs in size, can be cloned in cosmids. The cosmid can be propagated as a plasmid in vivo and packaged into bacteriophages in vitro.

cosmid

a class of plasmid-based vectors carrying the bacteriophage λ cos sequences required for packaging of DNA into phage particles. Used for cloning large DNA fragments (up to 45 kilobases). Recombinant molecules constructed using cosmids are incorporated into bacteriophage using in vitro packaging extracts and introduced with high efficiency into Escherichia coli.
References in periodicals archive ?
The positions of the CMT1A-REP sequences were confirmed on the BAC clones using sequences of the cosmid clone c74F4 for the proximal CMT1A-REP (17) and c15H12 for the distal CMT1A-REP sequence (31).
Larger gene fragments having 20-40 Kb size can be cloned in cosmids or fosmids.
Agrobacterium rhizogenes strain R1601 was obtained from the Plant Tissue Culture Laboratory /BIOAGRO/UFV and contains the plasmid pR and the cosmids pTVK 291 and 1500.
The strategies include subcloning genomic DNA from cosmids to plasmids, mutagenesis of that DNA and placing mutant DNA in the S.
Further work, including sequence analysis of the 5'--and 3'-untranslated regions of liver and leukocyte GRHPR cDNA and of cosmids containing GRHPR genomic DNA, is in progress.
CosMCPrep eliminates this problem, enabling the efficient, fully-automated purification of low copy plasmids such as BACs, cosmids and fosmids.
Phrap was originally developed with a more narrow focus as a tool to assist genome centers sequencing cosmids," stated Dr.
The procedure effectively amplifies even large DNA circles such as bacterial artificial chromosomes (BACs) and cosmids.
In addition to sequencing services, this product line now includes shotgun library construction from BAC/PACs, cosmids and whole genomes.