Three methyl-Co(III) methylamine-specific corrinoid
protein: CoM methyltransferase (Metho_0037, Metho_0007, Metho_0355) of M.
Holliger, "Characterization of the corrinoid iron-sulfur protein tetrachloroethene reductive dehalogenase of Dehalobacter restrictus," Applied and Environmental Microbiology, vol.
Diekert, "Tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans: substrate specificity of the native enzyme and its corrinoid cofactor," Archives of Microbiology, vol.
Many dechlorinators, such as the versatile Dehalococcoides, lack the ability to synthesize needed corrinoids for reductive dehalogenation and instead have genes for corrinoid scavenging and import [159,160].
Men et al., "Versatility in corrinoid salvaging and remodeling pathways supports corrinoiddependent metabolism in Dehalococcoides mccartyi," Applied and Environmental Microbiology, vol.
Rapid analysis of cobalamin coenzymes and related corrinoid
analogs by high-performance liquid chromatography.
The primer sequences that target genes coding for substrate-specific corrinoid proteins are shown in Table 1.
These corrinoid proteins are substrate specific and are referred to as MtaC, MttC, MtbC, and MtmC for the breakdown of methanol, TMA, DMA, and MMA, respectively.
one kb sequence preceding mtaCl (MA0456), which encodes the corrinoid
protein of the methanol-specific methyltransferase MT1 [35, 36].
The biological function of circulating HC is uncertain, but its ability to bind the so-called [B.sub.12] analogs (corrinoids
differing from active forms of [B.sub.12]) suggests that it might protect cells from possible toxicity of these analogs (13).
Vitamin [B.sub.12] is usually used as a generic term representing various cobalt-containing tetrapyrrole rings with attached nucleotide side chains that are chemically classified as cobalamins or corrinoids
. A corrin is the cobalamin tetrapyrrole ring that excludes cobalt and other side chains.
In contrast to the 2 other binding proteins, IF and TC, HC is characterized by its ability to bind both cobalamin and other corrinoids
, the so-called vitamin [B.sub.12] analogs (2).