After the collection of blood samples, piglets were immediately euthanized, and genital organs (ovary + cornu uteri + vagina-vestibule) were isolated, weighed, and gross lesions examined.
Ovary and cornu uteri tissues fixed in 10% buffered formalin were embedded in paraffin after routine processing.
ABSTRACT : This study was undertaken to investigate the distribution of major histocompatibility complex class II positive (MHC II+) (HLA-DP-like) cells in the cow uterus (cervix, corpus and cornu uteri) and to compare these cells between the estrus and diestrus phases of the estrous cycle.
Streptavidin-biotin peroxidase complex (StreptABC) staining was applied to three sections from each region of the uterus (cervix, corpus and cornu uteri) (Hsu et al., 1981; www.vmrd.com/docs/ab/ ihc.htm, 2001).
For each animal, three sections from the cervix, corpus and cornu uteri were scored.
In the surface epithelia of the cornu uteri, an MHC II+ reaction was seen in estrus but not in diestrus.
Endothelial cells also gave a positive reaction in the cervix, corpus and cornu uteri (Figure 4B).
Although the largest numbers of MHC II+ cells were found in the first region of the cornu uteri in estrus and the first region of the corpus uteri in diestrus, there were no statistically significant differences in cell numbers per [mm.sup.2] between estrus and diestrus in the cervix, corpus and cornu uteri (Table 2).
(1999) found that the numbers of MHC II+, CD4+ and CD8+ cells were higher in the uterine body than in the cornu uteri of mares during estrous, but the difference in MHC II cells was not significant.
In this study, tissue samples from the cervix, corpus and cornu uteri were examined, and MHC II+ cells were found in surface and glandular epithelia, in connective tissue surrounding the glands and in connective tissue areas outside the endometrium, surrounding the blood vessels.