Table 1 Showing the identification of various candida species by Hicrome and cornmeal agar
. In our study 10 different candida species were identified by each method.
The Candida isolates obtained were further identified by conventional methods such as gram stain (direct microscopy), germ tube test, Sabouraud dextrose broth, microscopic morphology on cornmeal agar
, and sugar assimilation tests.
Speciation of Candida using chromogenic and cornmeal agar
with determination of fluconazole sensitivity.
was purchased from Becton, Dickinson and Co, Sparks, MD, USA.
albicansa single colony of Candida was picked from the a pure culture medium and inoculated on a plate of Cornmeal agar
containing 1%Tween 80 and rice agar containing 0.8% Tween 80 by making 3 parallel cuts about half an inch apart at 45 degree angle to culture medium then a cover slip was added and incubated at 30C for 2 days.
Carbohydrate assimilation tests (ID32C system) were the second method of identification used instead of cornmeal agar
culture because the ID32C system has the capability to identify many Candida species (in excess of 30) and several non-Candida yeasts as well.
(1) The organism grows quickly on Sabouraud dextrose agar, blood agar, or cornmeal agar
as a septate, branched hyphal mold which may be difficult to morphologically discern from Aspergillus spp.
Cleistothecia were observed after 2 weeks of incubation on potato dextrose agar and cornmeal agar
(Figure 1, C).
 Germ-tube testing and morphology on cornmeal agar
were also performed to enable the accurate identification of Candida spp.
For fungal culture, specimen was inoculated on Sabouraud's agar and cornmeal agar
at 25 degree celsius as well as at 37 degree celsius.
The organism was subcultured on Cornmeal Agar
and brown-black cleistothecia containing numerous ascospores were observed following 14 days of incubation.