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complementary DNA (cDNA) (copy DNA (cDNA)) synthetic DNA transcribed from a specific RNA through the reaction of the enzyme reverse transcriptase.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.
complementary DNAa DNA molecule obtained in vitro from an RNA (usually eukaryotic mRNA) TEMPLATE, using the ENZYME REVERSE TRANSCRIPTASE. The DNA strand (cDNA) is complementary to the RNA. The RNA is removed after cDNA synthesis and the second cDNA strand is then synthesized using the enzyme DNA POLYMERASE to generate double-stranded DNA. cDNA thus represents double-stranded DNA copies of transcribed regions of a genome and, if using mature mRNA as template, will lack any INTRONS associated with the genomic region.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005