Table 1 List of taxa analyzed with novel genes and total number of contigs
after assembly Taxon Total contig
number Metazoa Porifera Kirkpatrickia variolosa (Kirkpatrick, 1907) 100,231 Latrunculia apicalis Ridley & Dendy, 1886 76,210 Ctenophora Coeloplana astericola Mortensen, 1927 222,614 Mnemiopsis leidyi A.
It should be noted that the quality of the de novo transcriptome assembly is reliant on the user-defined k-mer value characterized as the length parameter defining the sequence overlap between two reads forming a contiguous sequence (referred as a contig
Insertions were investigated using all contigs
>500 bp generated from de novo assembly of the reads unmapped after reference based assembly.
([greater than or equal to] 100 bp) 557,740 Large contigs
([greater than or equal to] 1,000 bp) 49,162 Maximum length (bp) 13,707 Average length (bp) 333.4 N50 (bp) 1,198 Contigs
after length filtering ([greater than or equal 156,686 to] 200 bp) Percentage contigs
kept after length filtering 28.09% Average contig
length after length filtering (bp) 984.2 Contigs
(After CD-HIT-EST + CAP3) 105,153 Average length (bp) (After CD-HIT-EST + CAP3) 591.2 Reads mapped to final reference (%) 70.62 TABLE 2.
For this clade a single contig
was then generated and deposited on NCBI GenBank under accession number JN797618.
(23) The final sequence for each strain was obtained using the Contig
Assembly Program (CAP) in BioEdit v.5.0.9 (22) and submitted to BLASTn for homology confirmation.
En la figura 5 se muestran los contig
que fueron analizados, asi como la descripcion de la banda a partir de la cual se realizo la clonacion y secuenciacion.
Fine genetic mapping and BAC contig
development for the citrus tristeza virus resistance gene locus in Poncirus trifoliata (Raf.).
Exonic splicing enhancer (ESE) finder (Cold Spring Harbor Laboratory, New York, US) was used to test whether these polymorphisms had the ability to affect or act as an ESE and found polymorphisms around several ESE motifs in the region (range contig
position from 21561001 to 21561901).
A 4 Mb high resolution BAC contig
on bovine chromosome 1q12 and comparative analysis with human chromosome 21q22.
SNP linked to Gene ABCB6(geneID:10058) Via Contig
Construction of a 350-kb sequence-ready 11q13 cosmid contig
encompassing the markers D11S4933 and D11S546: mapping of 11 genes and 3 tumor-associated translocation breakpoints.