contaminated culture


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contaminated culture

A culture in which bacteria from a foreign source have infiltrated the growth medium.
See also: culture
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Results Indirect Direct Direct LJ P (%) TTC MODS assay (%) assay (%) Valid test results Sensitive to both 189 (71.6) 184 (69.7) 183 (69.3) INH and RIF Resistance to INH 21 (8) 21 (8) 21 (8) and RIF Resistance to INH 9 (3.4) 10 (3.8) 11 (4.2) Resistance to RIF 2 (0.8) 3 (1.1) 4 (1.5) Sub total 221 (83.7) 218 (82.6) 219 (83) Invalid test results No growth 27 (10.2) 24 (9.1) 25 (9.5) Contaminated cultures 13 (4.9) 19 (7.2) 17 (6.4) Non-tuberculous 3 (1.1) 3 (1.1) 3 (1.1) mycobacteria Sub total 43 (16.3) 46 (17.4) 45 (17.1) INH, isoniazid; RIF, rifampicin; LJ PM, Lowenstein-Jensen proportion method; TTC assay, 2,3,5-triphenyl tetrazloium chloride assay; direct MODS assay, microscopic drug susceptibility assay.
(22) In a story previously detailed,7 the realization that HeLa cells had contaminated cultures so far afield led to a reappraisal of tissue culture stocks by the American Type Culture Collection (ATCC; Manassas, Virginia) and the Cell Culture Laboratory at the Naval Biosciences Laboratory (Oakland, California).